CHAPTER X. 



SERIAL SECTION MOUNTING. 



181. Choice of a Method. I recommend for general work 

 the following : For paraffin sections that do not require to 

 be flattened or stained, Schallibaunr's collodion. For paraf- 

 fin sections that are to be stained on the slide, the water 

 method, or Mayer's albumen. For collodion sections, 

 Mayers albumen. For very large collodion sections, Wei- 

 gert's process. 



Methods for Paraffin Sections. 



182. The Water Method. GAULE (Arch. f. Anat. u. Phys., 

 Phys. Abth., 1881, p. 156); SUCHANNEK (Zeit. f. iviss. Mik., 

 vii, 4, 1891, p. 464) ; GDLLAND (Journ. Anat. and Phys., 

 xxvi, 1891, p. 56) ; SCHJEFFERDECKER (Zeit. f. wiss. Mik., ix, 

 2, 1892, p. 202) ; HEIDENHAIN (Kern und Protoplasma, p. 

 114) ; NUSBAUM (Anat. Anz., xii, 2, 1896, p. 52) ; MAYER in 

 the Grundzuge, LEE und MAYER, 1898, p. 113; De GROOT 

 (Zeit. f. wiss. Mik., xv, 1, 1898, p. 62), and others (some ir- 

 rational variations have been suppressed). The principle 

 of this method is that the sections are made to adhere to the 

 slide by the mere molecular adhesion of intimate contact, 

 without the intervention of any cementing substance ; the 

 sections being brought into this intimate contact by being 

 slowly drawn down by the evaporation of a layer of water 

 on which they are floated. It is now practised, with un- 

 essential variations, as follows : 



(a] For sections that are large and not numerous. The 

 sections are flattened out on water by one or other of the 

 processes described in 138. The slide is then drained and 

 put away to dry until every trace of water has completely 



