184 CHAPTEE XIII. 



of stain. It is a perhaps even more precise nuclear stain, j 

 and the solution keeps better. 



240. MAYEE'S Glychsemalum (Mitth. Zool. Stat. Neapel, xii, 

 2, 1896, p. 310). Haematein (or hasmateate of ammonia) 

 0'4 grm. (to be rubbed up in a few drops of glycerin in a 

 mortar till it dissolves) ; alum, 5 grms. ; glycerin, 30 ; dis- 

 tilled water, 70. The stain is not purely nuclear, but may 

 be made so by washing out with alum solution or a weak 

 acid ( 236, 238). The solution keeps admirably. 



241. HANSEN'S Solution (Zool. Anz., 1895, p. 158). See last edition. 

 Hansen oxidises a mixture of alum and hsematoxylin by means of per- 

 manganate of potash. He fancies that by destroying all germs that may be 

 present in the ingredients, the permanganate should make the solution keep 

 better than the usual solutions. As a matter of fact it does not; mine 

 formed a pellicle and strong precipitate in a few days, and the same was 

 found at the Naples station (MAYER, in Hit.}. See further the remarks of 

 MAYER on this process in Mitth. Zool. Stat. Neapel, xii, 1896, p. 309, or the 

 Grundziige, p. 153. 



242. HARRIS'S Solution (Micr. Bull., xv, 1898, p. 47 ; Journ. Roy. Mic. 

 Soc., 1899, p. 236). Aluin-hsernatoxylin solution ripened by addition of 

 mercuric oxide. 



243. Bohmer's Haematoxylin (Arch.f. mik. Anat., iv, 1868, p. 345 ; 

 Aerzt. IntelligenzbL, Baiern, 1865, p. 382). Make (A) a solution of 

 hsematox. cryst. 1 part, alcohol (absolute) 12 parts and (B) alum 1 part, 

 water 240. For staining, add two or three drops of A to a watch-glassful 

 of B. 



The alcoholic solution of hsematoxylin ought to be old and brown ( 232)- 

 I consider this stain to "be of merely historical interest. 



244. Delafield's Heematoxylin (Zeit. f. wiss. Mik., ii, 1885, p. 288: 

 frequently attributed erroneously to GRENACHEROI- PRUDDEN). To 400 c.c. 

 of saturated solution of ammonia-alum* add 4 grms. of hsematox. cryst. 

 dissolved in 25 c.c. of strong alcohol. Leave it exposed to the light and air 

 in an unstoppered bottle for three or four days. Filter, and add 100 c.c. of 

 glycerin and 100 c.c. of methylic alcohol (CH 4 0). Allow the solution to 

 stand until the colour is sufficiently dark, then filter and keep in a tightly 

 stoppered bottle. 



This solution keeps well, it maybe said to keep for years. It is well to 

 allow it to ripen for at least two months before using it. 



For staining, enough of the solution should be added to pure water to 



* Ammonia-alum dissolves in about 11 parts water. 



