COAL-TAR CHROMATIN STAINS. 207 



Victoria has a special affinity for elastic fibres. For this 

 object Lustgarten recommends an alcoholic solution of the 

 dye diluted with two to four parts of water. Fixation in 

 chrom-osmium, or at least in a chromic mixture, is, I believe, 

 a necessary condition to this reaction. And you must stain 

 for a long time. 



Victoria has also a special affinity for mucus- cells, from 

 which it is not washed out by alcohol. 



MAGDALA RED (NAPTHALIN RED, ROSE DE NAPTHALINE). 



Fuchsin (meaning the basic fuchsins, a series of Rosanilin salts having 

 very similar reactions, and found in commerce under the names of FUCHSIN. 

 ANILIX RED, RUBIN, ROSEIN, MAGENTA, SOLFERINO, CORALLIN). GRASER 

 (Deutsche Zeit. f. Chirurgie, xxvii, 1888, pp. 538584; Zeit. f. wiss. Mil;., 

 v, 3, 1888, p. 378) stains for twelve to twenty-four hours in a dilute aqueous 

 solution, washes out for a short time in alcohol, stains for a few minutes in 

 aqueous solution of inethylen blue, and dehydrates with alcohol. A double 

 stain. Chromatin and nucleoli red, all the rest blue. 



ZIEHI/S Carbolic Fuchsin (from Zeit. f. iriss Mik., vii, 1, 

 1890, p. 39). 



The stain is made either by taking 



Fuchsin .... 1 gramme, 

 Acid, carbol. crist. . . 5 grammes, 

 Alcohol .... 10 



Aq. dest 100 



or by saturating a 5 per cent, aqueous solution of carbolic 

 acid with concentrated alcoholic solution of fuchsin (the 

 saturation of the carbolic solution with fuchsin is made mani- 

 fest by the formation of .a metallic -looking pellicle on the 

 surface of the liquid) . The stain is washed out with alcohol 

 followed by clove oil. 



Bismark Brown has this advantage, that being sufficiently resistent to 

 alcohol it may be utilised for staining entire objects. 



KAISER (Biblioth. Zool. H. 7, 1 Halfte, 1891 ; Zeit. f. wiss. Mik., viii, 

 3, 1891, p. 363) stains for forty-eight hours, and at a temperature of 60 C. 

 in saturated solution of Bismarck brown in 60 per cent, alcohol (the solution 

 to be made in boiling alcohol), and washes out (until all is decoloured except 

 the karyokinetic figures) in 60 per cent, alcohol, containing 2 per cent, 

 hydrochloric acid or 3 per cent, acetic acid. 

 METHYL VIOLET. 



BENZOAZURIN (MARTIN, Zeit.f. wiss. Mik., vi, 3, 1889, p. 193). Stain 

 for an hour or so in dilute aqueous solution, and wash out with HC1 alcohol. 

 METHYLEN BLUE. 



Nigrosin (RRERA Proc.-Verb. Soc. Beige de Mic., 1881, p. 134). The 

 stain resisjts alcohol well. 



