224 CHAPTER XVI. 



and eosin, of each two parts ; glycerin, thirty parts. This 

 gives a very thick, syrupy solution. To use it, cover- glass 

 preparations may be floated on to it ; or sections on slides 

 may have a few drops poured on to them, the slide being* 

 laid flat till the stain has taken effect (twenty-four hours 

 for Flemming material). This mixture was imagined for 

 the purpose of obtaining a specific stain of certain granules 

 of leucocytes. It has been pointed out by NIKIFOEOW (Zeit. 

 f. wiss. Mik., viii, 2, 1891, p. 189 ; and xi, 2, 1894, p. 246) 

 that it is also available for staining sections. I find this is 

 the case. With Flemming material it gives a powerful and 

 good stain, which is much more resistent to alcohol than that 

 of the EniiLiCH-BioNDi mixture, and is therefore much more 

 adapted to ordinary work. Chromatin in my preparations 

 is of a very dark blue, cytoplasm being of a lighter blue 

 (except where it has taken the stain of the aurantia or 

 eosin). It will thus be seen that the Indulin in this com- 

 bination behaves in a manner quite opposed to its behaviour 

 when used alone (see last ). The stain is said to keep 

 well. 



ISRAEL (Praktik. Path. Hist., Berlin, 1893, p. 68) gives a more compli- 

 cated receipt. 



310. Safranin and Indigo- Carmine or Nigrosin (KossiNSKi, Zeit. 

 f. wiss. Mik., vi, 1, 1880, p. 61). See previous editions. 



311. Anilin Blue. Under this title are comprised various 

 derivatives of the base rosanilin. They occur under the 

 names Spirit Soluble Blue (Bleu Alcool), Gentian Blue 6 B, Spirit 

 Blue 0, Opal Blue, Bleu de Nuit, Bleu Lumiere, Parma Blue, Bleu 

 de Lyon. Receipts of the older authors for staining with 

 " Anilin blue " should, I think, be disregarded, as it would 

 probably now be impossible to obtain the colours used by 

 them, or even to ascertain what colour was meant by them. 



The only one of the above-mentioned colours of which I 

 have any personal knowledge, or that appears really valuable, 

 is Bleu de Lyon. (Some authors give the names Bleu de Nuit 

 and Griinstichblau as synonyms of Bleu de Lyon.) I find 

 that though there is nothing very specific in its action, it is 

 a very good plasma stain. It is a fairly true plasma stain, 

 for though in a strong stain it will stain chromatin, yet in a 



