OTHER STAINS AND COMBINATIONS. 267 



383. Haematein and Saurefuchsin. Get a sharp chromatin 

 stain with iron-haematoxylin or haemalum, then stain (sections) 

 in 0*5 per cent, aqueous solution of Saurefuchsin, dehydrate 

 and mount. The time required for staining varies much with 

 the material, but is easily found by trial. 



384. Hsematoxylin and Saurefuchsin and Orange. Proceed 

 as above, using for the second stain the following mixture : 

 Saurefuchsin, 1 grm.; orange, 6 grms.; rectified spirit, 60 c.c.; 

 water, 240 c.c. (from SQUIRE'S Methods and Formulas, p. 42). 

 Using orange Gr (not mentioned by SQUIRE), I have had very 

 good results. 



The process described by CAVAZZANI (Riforma Med., 

 Napoli, 1893, p. 604; Zeit. f. iviss. Mik., xi, 3, 1894, p. 344) 

 is far too complicated to be recommendable. 



385. Haematoxylin and Picro-Saurefucnsin (VAN GIBSON, New 

 York Med. Journ., 1889, p. 57; quoted from MOELLER, Zeit. 



f. wiss. Mik., xv, 2, 1898, p. 172, which see for further 

 details). Proceed as above, using for the second stain the 

 picro- Saurefuchsin mixture, 289. The second stain must not 

 be too prolonged or the haematoxylin stain may be attacked. 

 This stain is now very much in vogue. 



386. Haematoxylin and Safranin. RABL (Morph. Jahrb., x, 1884, 

 p. 215) stained very lightly with vei'y dilute DELAFIELD'S hsematoxylin for 

 twenty-four hours, then for some hours in (PFITZNEB'S) safranin, and 

 washed out with pure alcohol. 



