MACERATION AND DIGESTION. 319 



in a few minutes. Epithelial cells of salamandra are said to 

 be dissociated instantaneously. 



Digestion. 



547. BEALE'S Digestion Fluid (Archives of Medicine, i, 1858, 

 pp. 296 316). The mucus expressed from the stomach 

 glands of the pig is rapidly dried on glass plates, powdered, 

 and kept in stoppered bottles. It retains its properties for 

 years. Eight tenths of a grain will dissolve 100 grains of 

 coagulated white of egg. 



To prepare the digestion fluid, the powder is dissolved in 

 distilled water, and the solution filtered. Or the powder may 

 be dissolved in glycerin. The tissues to be digested may be 

 kept for some hours in the liquid at a temperature of 100 F. 

 (37 C.). 



548. BRUCKE'S Digestion Fluid (from CARNOY'S Biologie cellu- 

 laire, p. 94). 



Glycerinated extract of pig's stomach . 1 vol. 

 0*2 per cent, solution of HC1 . . .3 vols. 

 Thymol, a few crystals. 



549. BICKFALVI'S Digestion Fluid (Centrabl.f. d. med. Wiss., 

 1883, p. 833). One grm. of dried stomachal mucosa is mixed 

 with 20 c.c. of 0'5 per cent, hydrochloric acid, and put into 

 an incubator for three or four hours, then filtered. Macerate 

 the tissue in the solution for not more than half an hour to 

 an hour. 



550. KUSKOW'S Digestion Fluid (Arch. f. mik. Anat., xxx, 

 p. 32; Zeit.f. wiss. Mik., iv, 3, 1887, p. 384). One part of 

 pepsin dissolved in 200 parts of 3 per cent, solution of oxalic 

 acid. The solution should be freshly prepared, and the 

 objects (sections of hardened Ligamentum Nuchae) remain in 

 it at the ordinary temperature for ten to forty minutes. 



551. SCHIEFFERDECKER'S Pancreatin Digestion Fluid (Zeit.f. 

 wiss. Mik., iii, 4, 1886, p. 483). A saturated solution of the 

 " Pankreatinum siccum," prepared by Dr. Witte, Eostock, is 



