354 CHAPTEE XXVI. 



636. Amphipoda. DELLA VALLE (Fauna u. Flora Golf. 

 Neapel, xx, Monog., 1893, p. 170) puts ova of Orchestia by 

 means of a pipette into boiling, cold-saturated sublimate solu- 

 tion, removes them instantly into sea water, and thence into 

 weak alcohol. If the chorion does not burst of itself it must 

 be pricked with a needle. 



637. Copepoda. HAECKER (Arch. f. mik. Anat., xlix, 1897, 

 p. 35) fixes ovisacs of Cyclops for ten minutes in VOM RATE'S 

 picro - platin-osmic mixture . 



638. Isopoda. McMuEEicn (Journ. of Morph., xi, 1895, 

 p. 65) fixes ova of Jaera in a saturated solution of picric acid 

 in 70 per cent, alcohol, to which has been added 2 per cent, 

 of concentrated sulphuric acid. If the yolk is brittle he 

 imbeds in celloidin ; if not, in paraffin. 



Vermes. 



639, Polychaeta. WILSON (Journ. of Morph., vi; 1892, 

 p. 373) stains living embryos of Nereis with methylen blue 

 in sea water. He fixes generally for ten to thirty minutes 

 with liquid of Flemming or Perenyi or Lang's sublimate ; 

 picro-sulphuric acid is not good. 



WISTINGHAUSEN (Mitth. Zool. 8 tat. Neapel, x, 1891, p. 47) 

 got his best results with advanced embryos by fixing for an 

 hour in a mixture of 1 part 1 per cent, osmic acid, 25 parts 

 1 per cent, chromic acid, 5 parts acetic acid, glacial, and 70 

 parts water, washing out for twenty-four hours in water and 

 bringing into 50 per cent, alcohol for three hours, then into 

 70 per cent. 



KOESCHELT (Zeit. f. wiss. Zool., Ix, 1895, p. 545) found 

 picro-acetic acid of Boveri the best thing for ova of Ophryo- 

 trocha : time, three to four hours, then 70 per cent, alcohol. 



KLEINENBEEG (ibid., xliv, 1886, p. 25) fixes larvae of Lopadorhynchus 

 with his picro-sulphuric acid, brings them into 70 per cent, alcohol, then 

 90 per cent., and stains with borax carmine. He also macerates them for 

 one or two hours in dilute picro-sulphuric acid, and then for twenty-four 

 hours in Beale's carmine. 



