CHAPTER XXVIII. 



TEGUMENTAEY ORGANS. 



653. Epithelium. Both for surface views and for sections 

 good results are obtained by the nitrate of silver method,, the 

 methylen blue method, the perchloride of iron and pyrogallol 

 method of the Hoggans, 362, the osmic acid and pyrogallol 

 process,, 361, and by iron-hsematoxylin. 



HEIDENHAIN'S fixative for intestinal epithelia may be useful 

 for soft epidermis ; it consists of a saturated solution of sali- 

 cylic acid in one-third alcohol (Sitz. Ber. Phys. med. G-es. 

 Wiirzburg, 26th January, 1899). 



For the purpose of separating the epidermis from the 

 corium, LOEWY (Arch. f. mik. Anat., xxxvii, 1891, p. 159 ; 

 Zeit. f. wiss. Mik., viii, 2, 1891, p. 222) recommends macerat- 

 ing for twenty-four to forty-eight hours, at a temperature of 

 about 40 C., in 6 per cent, pyroligneous acid. Acetic acid 

 of ^ per cent. (PHILIPPSON) is also good. 



For ciliated epithelium see the methods of Englemann 

 under " Mollusca." 



654. Intercellular Bridges (and Canals). On this subject 

 (which includes the so-called " Prickle-cells ") see the im- 

 portant memoirs of IDE, in La Cellule, iv, 2, 1888, p. 409, 

 and v, 2, 1889, p. 321; also KOLOSSOW, Arch. f. mik. Anat., 

 Hi, 1898, p. 1; Zeit. f. wiss. Mik., xv, 1, 1898, p. 92. 

 KOLOSSOW used an osmic-acid-tannin stain, 361 ; I would 

 suggest that iron-haematoxylin ought to be useful. 



See also FLEMMING, Anat. Hefte, 1 Abth., vi, 1895, p. 1. 



655. Keratohyalin. See UNNA, Monatsschr. prakt. Dermat., xx, 1895, 

 p. 69 ; Zeit.f. wiss. Mik., xiii, 1897, p. 337. 



