MUSCLE AND TENDON (NERVE-ENDINGS). 373 



The gold process gives positive images, the nervous structures 

 being stained dark violet. 



669. Nerve- endings the Bichromate of Silver Method. The 

 method of GOLGI has been successfully applied by RAMON Y 

 CAJAL to the study of the terminations of nerves and of 

 tracheae in the muscles of insects, and is doubtless susceptible 

 of still wider applications. The process used by him is the 

 rapid one. For details see Zeit.f. wins. Mik., vii, 3, 1890, p. 

 332, or previous editions. 



670. Nerve-endingsother Methods. See previous editions. 



Tendon. 



671. Corpuscles of Golgi (RANVIEE, Traite, p. 929). Take 

 the tendon of the anterior and superior insertion of the 

 gemini muscles of the rabbit. Free it as far as possible 

 from adherent muscle-fibres. Treat it according to the 

 formic acid and gold method ( 352), and after reduction of 

 the gold scrape the tendon with a fine scalpel, in order to 

 remove the muscle-fibres that mask the " musculo-tendinous 

 organs/' 



672. Corpuscles of Golgi (in the tendons of the motores 

 bulbi oculi) (VON MARCHES methods, Archivio per le Scienze 

 Hediche, vol. v, No. 15). The enucleated eyes, together 

 with their muscles, were put for not less than three days 

 into 2 per cent, bichromate of potash. The muscles and 

 tendons were then carefully dissected out, stained with gold 

 chloride and osmic acid (GoLGi's method), and by the 

 method of MANFREDI, 355. 



Mount all these preparations in glycerin (balsam clears 

 too greatly). The methods only succeed completely during 

 fine sunny weather. 



See also RUFFINI (Atti R. Ace. Lincei Roma Rend. [5], i, 1892, p. 442 ; 

 Zeit. f. wiss. Mik., ix, 2, 1892, p. 237), who recommends the method of 

 Fischer. 



