394 CHAPTER XXX. 



with solution of Miiller diluted with 2 parts of water, then for a quarter 

 of an hour with glycerin containing 1 or two drops of glacial acetic acid 

 for each cubic centimetre, and finally (without previous washing with 

 water) are stained for fifteen to twenty minutes in aqueous solution of 

 Ohina blue, washed out in alcohol, cleared in essence of turpentine, and 

 mounted in damar. 



PLATNEE'S Method (Zeit. f. wiss. Mik., vi, 2, 1889, p. 186). Small 

 nerves are fixed and hardened for several days in a mixture of 1 part of 

 Liq. Ferri Perchlor. (Ph. G., ed. 2) and 3 to 4 parts of water or alcohol, 

 washed out in water or alcohol till no traces of iron remain in them, 

 stained for several days or weeks in a concentrated solution of " Echtgriin " 

 in 75 per cent, alcohol, dehydrated, imbedded, and sectioned. See also 

 BEEE, Jahrb. Psychiatric, ii, 1893, 1 Heft. 



See also the papers of GEDOELST in La Cellule, iii, 1887, p. 117, and v, 

 1889, p. 126 (good details of digestion methods) ; also the report in Zeit. f. 

 wiss. Mik., vii, 1, 1890, p. 57. 



Cox (Anat. Hefte, i, H. 31, 1898, p. 75 ; Zeit. f. wiss. Mik., xv, 1899, 

 p. 369) fixes nerves in osmic acid of 2 per cent, (rabbit) or 1 per cent, 

 (frog), washes, dehydrates, clears with bergamot oil, and mounts in balsam. 

 The bergamot oil dissolves out the myelin, and leaves the neuroceratin 

 visible. It may be necessary to leave the nerves for forty-eight hours in 

 the oil. 



707. APATHY'S Neurofibrils (APATHY, Mitth. ZooL Stat. 

 Neapel, xii, 1897, p. 712), The Hsematein Method. 

 Material may be fixed with sublimate, liquid of Zenker, 

 picro-sulphuric acid, or any mixture that is not inimical to 

 staining with alum haematoxylin, and should be preserved in 

 90 per cent, alcohol. Portions are stained for at least forty- 

 eight hours in the haematein solution I A, 247, and are 

 then washed for up to twenty-four hours in absolutely pure 

 distilled water, preferably suspended therein. Before the 

 stain has become washed out of the neurofibrils, it is fixed 

 therein by putting the preparations for three to five hours 

 into spring water, after which they are put back for not 

 more than two hours into distilled water, dehydrated as 

 rapidly as possible by hanging them up in absolute alcohol, 

 and imbedded in paraffin, or celloidin, or glycerin jelly; 

 they must be protected from the light whilst in the chloro- 

 form through which they are passed into the paraffin, or 

 whilst in the celloidin. Sections are made and mounted in 

 a resin or in neutral glycerin. 



APATHY'S Gold Chloride After-gilding Method has been 

 given, 358 ; his Fore-gilding Method, 355, p. 255. 



