402 CHAPTER XXXI. 



is for young animals) begin to appear, washes in distilled water, and puts 

 into 1 per cent, oxalic acid until the non-medullated tracts appear light 

 brown, cortex and nuclei darker. Staining with carmine, etc., is not 

 necessary. 



713. KAISEE (Neurol. Centralb., xii, 1893, No. 11, pp. 364, 368; Zeit. 

 f. wiss. Mik., xi, 2, 1894, p. 249) hardens first in liquid of Miiller, then for 

 eight days in liquid of MARCHI ( 719), mordants sections with sesqui- 

 chloride of iron, stains, and differentiates with Pal's liquid. For details 

 see previous editions. 



Similarly BOLTON (Journ. of Anat. and Phys., xxxii, 1898, p. 245 ; 

 Zeit. f. wiss. MiJc., xv, 4, 1899, p. 457), who makes sections of formalin 

 material, and mordants them for a few minutes in 1 per cent, osniic acid, 

 or for a few hours in iron-alum or ammonium tnolybdate, stains in KULT- 

 SCHITZKY'S hsematoxylin (next ), and differentiates by Pal's process. 



Similarly to this, LASLETT (Lancet, 1898, p. 321; Journ. Hoy. Mic. 

 Soc., 1898, p. 600), who mordants in liquid of Marchi (1 week), makes 

 sections, stains by KULTSCHITZKY'S method, and differentiates by PAL'S. 



714. KULTSCHITZKY (Anat. Anz., 1889, p. 223, and 1890, 

 p. 519 ; Zeit. f. wiss. Mik., vi, 2, 1889, p. 196, and vii, 3, 1890, 

 p. 367) has given two modifications of WEIGERT'S method, of 

 which the following is the later : Specimens are hardened 

 for one or two months in solution of ErlicM, imbedded in 

 celloidin or photoxylin, and cut. Sections are stained for 

 from one to three hours, or as much as twenty-four, in a stain 

 made by adding 1 grm. of hsematoxylin dissolved in a little 

 alcohol to 100 c.c. of 2 per cent, acetic acid. They are 

 washed out in saturated solution of carbonate of lithia or 

 soda. 



Differentiation is not necessary, but by adding to the car- 

 bonate of lithia solution 10 per cent, of a 1 per cent, solu- 

 tion of red prussiate of potash, and decolourising therein for 

 two or three hours or more, a sharper stain is obtained. 

 After this the sections are well washed in water and mounted 

 in balsam. 



WOLTEES (Zeit. f. wiss. Mile., vii, 4, 1891, p. 466) proceeds as Kult- 

 schitzky, except that he stains in a solution kept warm by placing it on the 

 top of a stove kept at 45 C. for twenty-four hours, after which time the 

 sections are dipped in solution of Miiller, and differentiated by the method 

 of Pal. 



KAES (ibid., viii, 3, 1891, p. 388 ; Neurol. Centralb., 1891, No. 15) modi- 

 fies this by staining for as much as two or three days, and performing the 

 differentiation several times over. It appears doubtful whether either of 

 these modifications is an improvement. 



