NEUROLOGICAL METHODS. 403 



715. MITBOPHANOW (Zeit. f. wiss. Mile., xiii, 1896, p. 361) 

 mordants photoxylin sections for at least twenty-four hours 

 at 40 C. in a mixture of equal parts of saturated aqueous 

 solution of acetate of copper and 90 per cent, alcohol, stains 

 for ten minutes in Kultschitzky's haematoxylin, and differenti- 

 ates with Weigert's ferricyanide. Or after the copper bath 

 he stains for ten minutes in a solution of 1 grm. haematoxylin 

 in 400 c.c. of absolute alcohol with 4 c.c. of acetic acid, brings 

 into j per cent, solution of cyanide of potassium in 45 per 

 cent, alcohol until the photoxylin is discoloured, then into 

 the same with addition of 1 per cent, solution of red prussiate 

 of potash until the muscles are discoloured (this refers to 

 sections through the head of Anguilla). 



716. BERKLEY'S Rapid Method (N enrol. Centralb., xi, 9, 

 1892, p. 270; Zeit. f. wise. Mile., x, 3, 1893, p. 370). 

 Slices of tissue of not more than two and a half millimetres 

 in thickness are hardened for twenty-four to thirty hours in 

 mixture of Flemming , at a temperature of 25 C., then in 

 absolute alcohol, then imbedded in celloidin and cut. After 

 washing in water the sections are put overnight into a satu- 

 rated solution of acetate of copper (or they may be simply 

 warmed therein to 35 to 40 C. for half an hour). They 

 are then washed, and stained for fifteen to twenty minutes 

 in the fluid given below, warmed to 40 C., allowed to cool, 

 and differentiated for one to three minutes in Weigert's 

 ferricyanide liquid, which may be diluted if desired with 

 one third of water. Water, alcohol, bergamot oil, xylol- 

 balsam. 



The stain is made as follows : 2 c.c. of saturated solution of 

 carbonate of lithia are added to 50 c.c. of boiling water and 

 the solution boiled for two minutes more, when l\ to 2 c.c. 

 of 10 per cent, solution of haematoxylin in absolute alcohol 

 are added. 



This method is most suited to fresh material, and does 

 not give good results with tissues that have suffered post- 

 mortem changes. It suffers from the defective penetration 

 of the liquid of Flemming. 



Liquid of Flemming had been used before by FBIEDMA.NN (Neurol. 

 Centralb., 1885). 



