408 CHAPTER XXXI. 



The sections are then washed either in water or alcohol until the grey 

 matter can be clearly distinguished from the white, are cleared with cedar 

 oil, and mounted in balsam. Nerve tubes blue, ganglion cells greenish, 

 nuclei of neuroglia blue. 



727. Method of ADAMKIEWICS (Sitzb. k. Akad. Wiss. Wien. Math. 

 Naturw. KL, 1884, p. 245 ; Zeit. f. wiss. Mik., 1884, p. 587). Sections (of 

 spinal cord hardened in liquid of Muller for not less than one month and 

 not more than three) are washed first with water, then in water acidified 

 with a little nitric acid, and stained in concentrated solution of safranin. 

 They are then treated with alcohol and clove oil till no more colour comes 

 away, and are brought back again into water, washed in water acidified 

 with acetic acid, stained in methylen blue, and cleared as before. Myelin 

 red, nuclei violet. 



NIKIFOBOW (Zeit. f. wiss. Mik., v, 3, 1888, p. 338) impregnates with 

 gold chloride or other metallic salt after the safranin stain. 



Similarly CIAGLLNSKI (Zeit. f. wiss. Mik., viii, 1, 1891, p. 19) and 

 STEOEBE (ibid., x, 3, 1893, p. 336), both of them employing safranin 

 followed by anilin blue. 



For NISSL'S Congo red method see Zeit.f. wiss. Mik., iii, 1886, p. 398. 



728. FINOTTI (op. cit., 719) stains strongly in Delafield's hseinatoxylin, 

 then for a few seconds in concentrated solution of picric acid, then in 0'5 

 per cent. Saurefuchsin, and treats with alkaline alcohol (caustic potash). 



OHLMACHEE (Journ. Exper. Med., ii, 1897, p. 675) stains sections on the 

 slide for one minute with anilin-water gentian, 272, then for a few 

 seconds in a solution of 0*5 per cent, of Saurefuchsin in saturated solution 

 of picric acid diluted with one volume of water, washes well with water, 

 differentiates with alcohol and clove oil, and mounts in balsam. 



729. ARONSON (Centralb. med. Wiss., 1890, p. 577) stains sections of 

 material hardened in liquid of Erlicki or Muller (these must be mordanted 

 with acetate of copper) for twelve to twenty-four hours in a solution of 

 3 to 4 c.c. of Gallein (Griibler & Co.) in 100 c.c. of water with 20 of 

 alcohol and three drops of concentrated solution of carbonate of soda. 

 They are then differentiated by the method of Weigert, or Pal, or with 

 chloride of calcium, 717, then brought into concentrated solution of 

 carbonate of soda or lithia until they become red, and are mounted in 

 balsam (clear with oil of origanum). Nerve fibres red. A second stain 

 with methylen blue may follow after differentiating with permanganate. 



