NEUROLOGICAL METHODS. 483 



Path., i, 1888, p. 1 ; Anat. Am., 1889, p. Ill; Zeit. f. wias. Mik. t v, 3, 

 1888, p. 373, and vi, 2, 1889, p. 204. 



COLUCCI (Zeit.f. wiss. Mile., xii, 1, 1895, p. 87) recommends Paladino's 

 iodide of palladium impregnation, 725. 



For Bleaching see 575 583. 



781. Sections. Some workers recommend celloidin ; but I 

 consider paraffin preferable. Sections may be mounted in 

 dammar or (FLEMMING) in glycerin. 



782. Dissociation Methods. For maceration preparations 

 you may use weak solutions (0'2 to 0*5 per cent.) of osmic 

 acid for fixation, and then macerate in 0*02 per cent, chromic 

 acid (M. SCHULTZE), or in iodised serum (M. SCHULTZE), or in 

 dilute alcohol (LANDOLT), or in Miiller's solution, or (RANVIER, 

 Traite, p. 957) in pure water, for two or three days. THIN 

 (Joum. of Anat., 1879, p. 139) obtained very good results 

 by fixing for thirty-six to forty-eight hours in one third 

 alcohol, or in 25 per cent, alcohol, and then staining and 

 teasing. 



SCHIEFFERDECKER macerates fresh retina for several days in 

 the methyl mixture, 545. 



KRAUSE (Intern. Monatssch. f. Anat. u. Hist., 1884, p. 225 ; 

 Zeit.f. wiss. Mik., 1885, pp. 140, 396) recommends treatment 

 for several days with 10 per cent, chloral hydrate solution ; 

 the rods and cones are well preserved. 



Inner Ear. 



783. SCBWALBK (Beitr. z. Phys., 1887 ; Zeit. f. wiss. Mik., 

 iv, 1, 1887, p. 90; Journ. Roy. Mic. Soc., 1887, p. 840). Fix 

 (cochlea of guinea-pig) for eight to ten hours in " Flemming," 

 wash in water, decalcify (twenty-four hours is enough) in 1 

 per cent, hydrochloric acid, wash the acid out, dehydrate, and 

 imbed in paraffin. See also FERRBRI, 573 (phloroglucin) . 



PRENANT (Intern. Monatsschr. f. Anat. u. Physiol., ix, 1, 

 p. 6; Zeit. f. wiss. Mik., ix, 3, 1893, p. 379). For sections, 

 open the cochlea in solution of Flemming or of Hermann, 

 and fix therein for four to five hours. Avoid decalcification 

 as far as possible, as it is inimical to good preservation of 



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