CHAPTER XXXIII. 



SOME OTHER HISTOLOGICAL METHODS. 



Connective Tissues. 



786. Connective Tissue. S. MAYEE (Sitzb. ~k. Akad. Wiss., 

 Ixxxv, 1882, p. 69) recommends for staining fresh tissue a 

 solution of 1 gramme of " Violet B " (Bindschedler and 

 Busch, Bale) in 300 c.c. of 0*5 per cent, salt solution. 

 Elastic fibres and smooth muscle also stain, but of different 

 tints. 



For RANVIER'S method of artificial oedemata for the study 

 of areolar tissue, see his Traite, p. 329. 



FREEBORN (Amer. Mon. Mic. Journ., 1888, p. 231; Jourti. 

 Roy. Mic. Soc.j 1889, p. 305) recommends (for sections) picro- 

 nigrosin, made by mixing 5 c.c. of 1 per cent, aqueous solu- 

 tion of nigrosin with 45 c.c. of aqueous solution of picric 

 acid. Stain for three to five minutes, wash with water, and 

 mount in balsam. 



VAN GIBSON'S Picro- Saurefuchsin Stain has been given, 

 289. 



RAMON Y CAJAL recommends 0*1 grm. of Saurefuchsin to 

 100 of saturated solution of picric acid (SCHAFFER, Zeit. wiss. 

 Zool., Ixvi, 1899, p. 214; Zeit. f. wiss. Mik., xvi, 4, 1900, 

 p. 464). 



HANSEN (Anat. Anz., xv, 1898, p. 152) adds 5 c.c. of 2 per 

 cent, solution of Saurefuchsin to 100 c.c. saturated solution 

 of picric acid, and for staining adds to 3 c.c. of the mixture 

 one third of a drop of 2 per cent, acetic acid, stains for a 

 few minutes or hours, rinses in 3 c.c. of water with 2 drops 

 of the acidified stain added, dehydrates, clears with xylol, 

 and mounts in xylol-balsam. Connective tissue red, elastin 

 and all other elements yellow. 



