438 . CHAPTER XXXIII. 



but the plasma cells, and may then be mounted in resin-tur- 

 pentine solution. 



Mucus cells and fat cells are also sometimes stained by 

 these solutions. 



Other Media. In a similar way other soluble anilins may 

 be employed (in the form of a fluid containing 7^ per cent. 

 of acetic acid), primula, iodine violet, methyl violet, pur- 

 purin, safranin, fuchsin ; of these, methyl violet gives the 

 best results. 



See also SCHIEFFERDECKER and KOSSEL'S Gewebelehre, p. 329. 



793. Plasma Cells (NqfrDMANN, Beitr. z. Kenntniss d. Mast- 

 zellen, Inauguraldiss., Helmstedt, 1884). NOBDMANN finds it 

 useful to employ a concentrated solution of vesuvin contain- 

 ing 4 to 5 per cent, of hydrochloric acid. Sections should 

 remain for a few minutes in the solution, and then be de- 

 hydrated with absolute alcohol. The paper quoted contains 

 a detailed discussion of the micro-chemical reactions of 

 granule cells. 



794. Plasma Cells and Mastzellen. UNNA, Zeit.f. wiss. Mik., 

 vii, 4, 1892, p. 475, gives the following : 



A. For Plasma Cells. 



Methylen blue - r - . 3 c 1*0 



Caustic potash itw?.8 V; 0'05 



Distilled water *';:; . . lOO'O 



Add a few drops of this to ten, fifty, or one hundred vols. 

 of anilin water (p. 203) in a watch glass, and stain (alcohol 

 material, or at most sublimate and alcohol material, not 

 chromic material) for half an hour, several hours, or over- 

 night. Dehydrate rapidly in absolute alcohol, differentiate 

 in creosol (details not given), rinse in xylol, and mount in 

 balsam. 



B. General Stain, also bringing out Plasma Cells. 



, Methylen blue . . . .1*0 



Carbonate of potash . .1*0 



Distilled water '. . . lOO'O 

 Alcohol 20-0 



