444 CHAPTER XXXIII. 



For a similar method of KUPRECHT, see Zeit. /. wiss. Mile., xiii, 1, 1896, 

 p. 21, wherein see also quoted (p. 23) a method of ZIMMEEMANN. 



802. Sections of Bones or Teeth showing the Soft Parts. 

 NEALEY (Amer. Mo it. Mic. Journ., 1884, p. 142 ; Journ. Roy. 

 Mic. Soc., 1885, p. 348) says that perfectly fresh portions of 

 bone or teeth may be ground with emery on a dentist's lathe, 

 ;and good sections, with the soft parts in situ, obtained in 

 half an hour. 



HOPKWELL-SMITH (Journ. Brit. Dent. ASK., xi, 1890, p. 310 ; 

 Journ. Roy. Mic. Soc., 1890, p. 529) says that for preparing 

 sections of teeth showing odontoblasts in situ the best plan is 

 to take embryonic tissues. A lower jaw of an embryonic 

 kitten or pup may be taken, and hardened in solution of 

 Miiller followed by alcohol, then cut with a freezing micro- 

 tome. The knife cuts quite easily the thin cap of semi- 

 calcified dentine and bone. 



WEIL (loc. cit., 175) fixes pieces of fresh teeth in subli- 

 mate, stains with borax-carmine, brings them through 

 alcohol into chloroform and chloroform- balsam, and after 

 hardening this by heat proceeds to grind as usual ( 172). 



See also EOSE, last . 



803. VIVANTE (Intern. Monatssch. f. Anat. u. Phys., ix, 1892, p. 394; 

 Zeit. f. wiss. Mile., ix, 3, 1893, p. 351) has made out that thin specimens of 

 bone can be successfully treated by Golgi's bichromate of silver process. He 

 places portions of frontal bone of four to six months calves, which are not 

 more than 3 to 4 mm. thick, for eight days in solution of Miiller, th.en in the 

 osmium bichromate mixture, and then in the silver solution. After impreg- 

 nation the specimens should be decalcified, which may be done by putting 

 them for twenty days into von Ebner's mixture ( 563), after which they 

 should be well washed with water and brought into solution of carbonate of 

 soda, and finally imbedded in paraffin. Tor his quinolein blue method, see 

 last edition. 



For UNDERWOOD'S gold process for teeth, and for that of LEPKOWSKY, 

 see third edition. 



For the study of the vessels in teeth, LKPKOWSKY (Anat. 

 Hefte, viii, 1897, p. 568) injects with Berlin blue, hardens 

 the teeth with a piece of the jaw for one or two days in 50 

 per cent, formol, decalcifies in 10 per cent, nitric acid (eight 

 to fourteen days, change frequently) and makes celloidin 

 sections. 



