SOME OTHER HISTOLOGICAL METHODS. 449 



solution (MAX FLESCH, Zeit. f. wiss. Mik., v, 1, 1888, 

 p. 83). 



GRIESBACH also (op. cit., p. 328) prefers osmic acid, not 

 only as being a first-rate fixing agent, but because it can be 

 combined with certain stains without decomposing them. 

 He mentions methyl green, methyl violet, crystal violet, 

 safranin, eosin, Saurefuchsin, rhodamin, and iodine in potassic 

 iodide. 



Eossi (Zeit. f. wiss. Mik., vi, 4, 1889, p. 475) advises a 

 mixture of equal parts of 1 per cent, osmic acid, water, and 

 strong solution of methyl green, permanent mounts being 

 made by means of glycerin cautiously added. 



EWALD (Zeit. Biol., xxxiv, 1897, p. 257) mixes three to 

 four drops of blood of amphibia or reptiles with 10 c.c. of a 

 solution of 0*5 per cent, osmic acid in 0'5 per cent, salt 

 solution (for mammals 0*6 to 0*7 per cent, salt), siphons off the 

 supernatant liquid after twenty-four hours with his capillary 

 siphon ( 3, p. 4), and substitutes water, alum-carmine, etc., 

 and lastly, 50 per cent, alcohol. 



See also ABNOLD, Arch. path. Anat., cxlviii, 1897, p. 479. 



The mercurial liquids of Pacini ( 405) used to be con- 

 sidered good. HAYEM (op. cit.; see also Zeit. f. wiss. Mik., 

 vi, 3, 1889, p. 335) has the following formula : sublimate 

 0'5, salt 1, sulphate of soda 5, and water 200. This should 

 be mixed with blood in the proportion of about 1 : 100. 

 Eosin may be added to it. Lowrr's formula (Sitzb. k. Akad. 

 Wiss. Wien, xcv, 3, p. 129 ; Zeit. wiss. Mik., vi, 1, 1889, 

 p. 75) consists of 5 c.c. cold saturated sublimate solution, 5 

 grms. sulphate of soda, 2 grms. salt, and 300 c.c. water. 

 Mosso finds, however, that both of these are too weak in 

 sublimate. 



Of course other well-tried fixing fluids, such as Flemming's 

 solution, or Hermann's, may also be used for blood. 



LAYDOWSKY (Zeit. f. wiss. Mik., x, 1, 1893, p. 4) describes some remark- 

 able results obtained by fixing with 2 per cent, iodic acid, and staining with 

 Neu-Victoriagriin, methyl violet 6s, or gentian violet, a process which is 

 said to reveal the presence of nuclei in elements generally considered to be 

 apyrenematous. 



DUBOSCQ (Arch. Zool. Exper., vi, 1899, p. 481 ; Journ. 

 Roy. Mic. Soc., 1899, p. 545) uses (for blood of Chilopoda) 



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