SOME OTHER HISTOLOGICAL METHODS. 451 



(The methyl violet is to be dissolved in the glycerin with 

 one half of the water added to it ; the two salts are to be 

 dissolved in the other half of the water, and the two solu- 

 tions are to be mixed and filtered.) White blood-cor- 

 puscles stain in this medium in five or ten minutes; the 

 maximum of coloration is attained in from twenty to thirty 

 minutes. White blood- corpuscles, violet ; red blood-cor- 

 puscles, greenish. 



Ferrier's liquid is said to have a sp. gr. similar to that of 

 liquor sanguinis. Fuchsin, 1 grm. ; water, 150 c.c. ; rectified 

 spirit 50 c.c. ; dissolve, and add glycerin, 200 c.c. (from 

 SQUIRE'S Methods and Formula, p. 39). 



For UNNA'S methods for staining erythrocytes in alcohol-hardened 

 tissues (pathological extravasations) see Ze.it. /. wiss. Mik., xiii, 1896, p. 

 234. 



GiGLio-Tos (Zeit. f. iviss. Mik., xiv, 1897, p. 359) stains 

 films for one minute in a warm saturated solution of methylen 

 blue B.X., and examines in water. 



He later (ibid.., xv, 1898, p. 166) mixes fresh blood with 

 a saturated solution of neutral red in 0*8 per cent, salt 

 solution, which stains hasmoglobigenous granules in five to 

 ten minutes. 



Neutral red is also recommended as an intra vitam stain 

 for granules of leucocytes by EHRLICH and LAZARUS, see 

 296. 



ZIEMANN (Zeit. f. iciss. Mik., xv, 1899, p. 456) stains films 

 for twenty minutes in a mixture of 1 c.c. of 1 per cent, 

 methylen blue and 5 c.c. of 0*1 per cent, eosin solution 

 (mark B.A. or A.G., Hochst). 



JENNKR'S mixture, see 807. 



PRINCE (Micr. Bull., xv, 1898, p. 42 ; Joarn. Roy. Mic. Soc., 

 1899, p. 237 ; Zeit. f. iciss. Nik., xvi, 4, 1900, p. 468) adds 

 to twenty-four parts saturated solution of toluidin blue one 

 of saturated solution of Saurefuchsin and two of 2 per cent, 

 eosin, agitates and decants. Films are stained in a few 

 seconds in the fresh solution, or in a few minutes after it 

 has stood for some weeks. 



It goes without saying that the Ehrlich-Biondi mixture, 

 ( 290), and Ehrlich's triacid and acidophilous mixtures, 

 ( 291 and 309) may be found valuable in many haemato- 

 logical researches. 



