SOME OTHEK H1STOLOGICAL METHODS. 455 



mixture has become quite dark. Stir thoroughly. The 

 liquid having become thick, add a little 50 per cent, alcohol, 

 in which the warm mass ought to dissolve easily, and rinse 

 the whole with more alcohol into a bottle. Make up to 100 

 c.c. with 50 per cent, alcohol, let it stand for at least 

 twenty-four hours, and filter. This gives a stock solution, 

 which is as a rule to be diluted for use tenfold with distilled 

 or tap water. Exceptionally it may be diluted instead five 

 or ten fold with alcohol of 50 per cent, or 70 per cent. 

 The stock solution may be obtained from Griibler and Co, 

 Mucicarmine stains in sections or thin membranes mucus 

 only. Nuclei may be stained before with haemalum. 



813. MAYER'S Muchaematein (ibid.). Haematein 0'2 gramme, 

 aluminium chloride O'l gramme, glycerin 40 c.c., water 60 c.c. 

 Eub up the haematein in a mortar with a few drops of the 

 glycerin, then add the other ingredients. If it be desired 

 to avoid employing a watery liquid, an alcoholic solution 

 may be made in the same way by dissolving the haematein 

 and aluminium chloride in 100 c.c. of 70 per cent, alcohol, 

 with or without the addition of two drops of nitric acid. 

 This is a nearly pure mucus xtain for sections or thin 

 membranes. Nuclei may be stained before with paracar- 

 mine. 



If the mucus swells much (as in Fishes) the alcoholic solutions of inuci- 

 carmine or muchsematein are indicated, and watery fixatives should be 

 avoided as much as possible, 



814. Mucicarminic Acid (RAWITZ, Anat. Anz., xv, 1899, 

 p. 439). 1 gramme of carminic acid, 2 of aluminium chloride, 

 and 100 c.c. of 50 per cent, alcohol are dissolved and 

 evaporated to dryness on a sand-bath and the residue taken 

 up with 100 c.c. of 50 per cent, alcohol. Use as muci- 

 carmine. 



815. Neutral Red. KULTSCHIZKY (Arch. mik. Anat., xlix, 1897, p. 8) 

 fixes in his mixture (end of 59), and stains sections either in safranin with 

 2 percent, acetic acid, or in a similar solution of neutral red (two. to three 

 days, washing out with alcohol). 



816. Goblet Cells. So far as these contain mucin they give 

 the reactions above described, see FLEMMING, Zeit. f. wiss. 



