156 CHAPTER XXXIII. 



Mik., 1885, p. 519; PAULSEN, ibid., p. 520; PANETH, Arch, 

 f. mik. Anat., xxxi, 1888, p. 113 et seq. ; and LIST, ibid., 

 xxvii, 1886, p. 481. 



RANVIEE (Comptes rend., 1887, 3, p. 145 ; Zeit.f. wiss. Mik., v,2, 1888, 

 p. 233) treats the pharyngeal mucosa of the frog first for ten or twelve 

 hours with vapour of osmium, and then for three minutes with vapours of 

 perruthenic acid (Ru0 4 ) and obtains the mucigen in the goblet cells stained 

 black. 



817. Salivary Glands. SOLGER (Unters. z. Naturlehre d. 

 Menschen, xv, 5 and 6, pp. 2 15; Festschr. f. Gegenbaur, 

 ii, 1896, p. 211; Zeit. f. wiss. Mik., xii, 3, 1896, p. 374) 

 demonstrates the granules in serous cells and half-moons of 

 the submaxillary gland by means of formaldehyde. The 

 gland is hardened for two days or more in a 10 per cent, 

 solution of formol, and may then either be sectioned and 

 examined in the wet way or imbedded in paraffin, and the 

 sections stained with hsematoxylin of Delafield or of Ehrlich, 

 the granules taking the stain. 



KKAUSE (Arch. f. mik. An at., xlv, 1895, p. 94) stains 

 sections either with Heidenhain's iron hsematoxylin or with 

 Ehrlich-Biondi mixture or thionin. See also KRAUSE, ibid., 

 xlix, 1897, p. 709. 



818. Glands of Larynx and Trachea. FUCHS-WOLFRING (Arch, 

 mik. Anat., Hi, 1898, p. 735 ; Zeit. f. wiss. Mik., xv, 2, 1898, 

 p. 232) found mucicarmine the best reagent for mucus. It 

 is advantageous to employ it with haemalum ( 812). He 

 also used a light stain with Delafield's hsematoxylin followed 

 by Congo red (demonstrates the secretory capillaries of 

 serous glands). 



819. Gastric Glands. KOLSTER (Zeit. f. wiss. Mik., xii, 

 1895, p. 314) differentiates the two kinds of cells in stomach 

 glands by over-staining with heematoxylin, washing out with 

 alcohol containing 1 per cent, of HC1, blueing with alcohol 

 containing 1 per cent, of ammonia, and, after washing, 

 staining for one to five minutes in a weak solution of 

 Saurefuchsiii. Peptic cells blue, parietal cells red. Osmic 

 material cannot be employed. 



See also OPPEL, Lehrb. Vergl. Anat. Wirbelthiere, 1, Der Magen, Jena,. 

 1896. 



