468 CHAPTER XXX IV. 



KOSENSTADT (Arch, mik. Anat., xlvii, 1896, p. 748) fixes eyes, 

 of Decapods in a warm mixture of three parts concentrated 

 sublimate solution and one part liquid of Perenyi, and de- 

 pigmentates them in a mixture of three parts each of nitric 

 and hydrochloric acid and 100 of water, warmed to 56 C. 

 for a few hours. 



VIALLANES (Ann. Sci. Nat., xiii, 1892, p. 354; Journ. Roy. 

 Mic. Soc., 1893, p. 260) fixes eyes of Palinurus in 5 per cent, 

 sublimate with 5 per cent, acetic acid, washes out in 70 per 

 cent, alcohol, depigmentates in a mixture of equal parts of 

 alcohol, glycerin, and water, through which chlorine gas is. 

 led, puts for twelve hours into 1 per cent, solution of cupric 

 sulphate, washes for five to six hours in distilled water, and 

 stains for twelve hours in a fresh solution of one part haema- 

 toxylin in 100 of absolute alcohol and 300 of distilled water. 

 He then puts them back for the same time into the copper 

 solution, washes, and passes through alcohol and makes 

 paraffin sections. The sections may be afterwards stained 

 with safranin. 



839. Brain of Bees. KENYON (Journ. Comp. NeuroL, vi 

 1896, p. 137; Journ. Roy. Mic. Soc., 1897, p. 80) treats them 

 by the G-OLGI process (seldom successful), or hardens in a 

 mixture of one part formol and two of 5 per cent, sulphate of 

 copper, followed by staining in Mallory's phospho-molybdic 

 haematoxylin, 259. 



840. Ventral Cord. BINET (Journ. de I' Anat. et de la Phys., 

 xxx, 1894, p. 469) fixes the ganglia of Hexapods either in 

 liquid of Flemming, or in Viallanes's sublimate, 838, treats 

 them with his copper hsematoxylin, 838, and makes paraffin 

 sections, which he stains with safranin. 



841. Injections (Arachnida and Crustacea especially). 

 AIME SCENEIDEE (Toblettes Zool., ii, ]892, p. 123) recom- 

 mends lithographic Indian ink, the animals being narcotised 

 with chloroform, then injected and thrown into strong 

 alcohol. 



CAUSARD (Bull. Sc. France Belg.,xxix, 1896, p. 16) injects 

 spiders with Indian ink, brings them into alcohol, and thence 

 for at least a day into water containing a few drops of 



