494 CHAPTER XXXIV. 



BRANDT (Fauna u. Flora Golf. Neapel,xiii, 1885, p. 7; Journ. 

 Roy. Hie. Soc., 1888, p. 665) fixes Sphserozoa, according to the 

 species, either with chromic acid of 0'5 per cent, to 1 per 

 cent, (half an hour to an hour), or with a mixture of equal 

 volumes of sea water and 70 per cent, alcohol with a little 

 tincture of iodine for a quarter to half an hour, or with a 

 5 to 15 per cent, solution of sublimate in sea water. 



Sporozoa. WASIELEWSKI (Sporozoenkunde, Jena, 1896, p. 

 153) lays great stress on the study of the living organisms, 

 either in their natural medium, or in normal salt solution, or 

 in a medium composed of 20 parts white of egg, 200 of water, 

 and 1 of common salt. He fixes Gregaringe and Coccidia 

 with osmic acid, sublimate, or picro-sulphuric acid, and 

 Myxosporidia with liquid of Flemming. He stains G-regarinae 

 with safranin, picro-carmine, etc., besides employing gold 

 chloride, silver nitrate, acetic acid, ammonia, etc., and 

 Myxosporidia with safranin or gentian and eosin. 



See also the methods of FABRE-DOMERGUE, Ann. de Microgr., ii, 1889, 

 p. 545, and 1890, p. 50 ; SCHEWIAKOFF, Biblioth. ZooL, v, 1889, p. 5 ; 

 Journ. Roy. Hie. Soc., 1889, pp. 832, 833 ; ZOJA, Boll. Sci. Pavia, 1892 ; 

 Zeit. f. wiss. Mik., ix, 4, 1893, p. 485 ; LONGHI, Bull. Mus. Zool. Univ. 

 Genova, 4, 1892; Zeit. f. wiss. Mik., ix, 4, 1893, p. 483; LAUTERBORN, 

 Zeit. wiss. Zool., lix, 1895, p. 170; SCHAUDINN, ibid., p. 193; BALBIANI, 

 Zool. Anz., xiii, 1890, p. 133; KARAWAIEW, ibid., xviii, 1895, p. 286. 



882. Sections. Sections of the larger Protozoa, and 

 amongst them of the larger forms of Infusoria (Stentor, 

 Bursaria, Nyctotherus), may be obtained without much diffi- 

 culty. The organisms should be strongly fixed, then dehy- 

 drated and cleared, and brought into melted paraffin in a 

 small watch glass. After a few minutes therein they are 

 brought on a cataract needle on to a small block of paraffin, 

 and arranged there with a heated needle (p. 93) and sec- 

 tioned. They may be stained after fixation, or the sections 

 may be stained on the slide, 182 or 183. 



LAUTEKBORN (loc. cit. last ) brings the objects through 

 chloroform into paraffin in a small glass tube, and after 

 cooling breaks the tube and so obtains a cylinder of paraffin 

 with the objects ready for cutting, 



HOYER (Arch. mik. Anat., liv, 1899, p. 95) performs all 

 the operations in a glass cylinder (5 cm. long and 7 'mm. 

 wide) open at both ends, but having a piece of moist parch- 



