same reagents in the same bottles or disltes, front lli<- 

 first Jixing fluid to the solid parafin. And from this 

 point the two are cut at the same stroke of the micro- 

 tome knife, fastened to the slide together, stained 

 together, and appear side by side in the same field of 

 the microscope. 



All this was made easy by a simple device, which 

 may be of use to others. The ganglia were usually 

 left attached to their segment of the spinal cord until 

 ready to pass into strong alcohol. They are then 

 trimmed for cutting, and arranged in the same posi- 

 tion relative to each other upon one end of a small 

 strip of mica; 1x3 cm. is a convenient size. As they 

 lie upon the slip, a drop of the thin white of an egg is 

 placed over them. This is allowed to dry somewhat 

 and the whole carefully laid in the alcohol, where the 

 egg speedily coagulates, holding the ganglia firmly to 

 each other and to the mica slip. The rule of always 

 placing the stimulated ganglion nearest the end of the 

 slip aids in simplifying matters. Any desired record 

 may be scratched upon the other end of the slip. 

 Not only one, but several pairs can be fastened to the 

 same slip, arranged in a row so that they all may be " 

 cut at the same time. For example, it was my prac- 

 tice to stimulate the right brachial and sciatic plex- 

 uses of a frog. This places at our disposal five pairs of 

 ganglia. These may be hardened by five different 

 methods, and all be arranged as described above on a 

 single slip of mica. They are all cut together, fastened 

 to the slide together (invariably by the alcohol fixing 

 method where differences of staining or granulaticm 

 are to be studied), and all stained together. Many 

 slides are obtainable from one such set of ganglia, and 

 each slide may be stained in a different way. This 



