10 iiodge: 



ulated for a loiujer or .•shorter tiiiic, ivhilc llie otiicr hos 



not. Methods of hardening and staining do not con- ^ 



cern us so long as the two ganglia go through every 



step of the processes together. : 



For the encouragement of others I may say lliat I | 



have tried almost every method practicable and 



impracticable, in the hope of finding a striking rear- i 



tion. Some such were found, but up to date they 

 have all proved inconstant. Trzebinski^ has made a 



special study of the influence of hardening reagents . 



upon the ganglion cells of the spinal cord. He finds I 



corrosive sublimate one of the best reagents, and states 



that it does not produce vacuolation of the cell. This | 



method followed by Gaule's quadruple staining has | 



furnished my best preparations for the study of granu- | 



lation and staining. Trzebinski did not, it wouhl > 



seem, experiment with osmic acid. This, witli fuehsiii, 



safranin, or all four of the Gaule stains, has given a | 



most beautiful preservation of the form of the nucleus 

 and the minute structure of the cell protoplasm. 



Two widely different animals, the frog and cat. ; 



were purposely selected to furnish the material for 

 investigation. And the results which I will now con- 

 sider are derived from fifteen experiments upon frogs 

 and ten or eleven experiments upon cats. -4// th>^ 

 experiments will be referred to either singly or in 

 groups. 



Results. 



Frog No. 1 was given three drops of 1 per cent 

 curare solution, and the sciatic nerve of the right side j 



was stimulated continuously for thirty minutes. The 



' Einiges iiber die Einwirkung der Hiirtungsmethoden auf die 

 Be.schaffenheit der Ganijlienzellen im Riickenmark der Hunde und 

 Kaninchen. Trzebinski. Vircliow's Arciiiv, Vol. 107. 



