ELECTRICALLY STIMULATED OANOLIf)N CELLS. 11 



three pairs of sciatic ganglia were excised and, with 

 those of a control frog, hardened in corrosive sub- 

 limate. The ninth pairs were stained in toto in soda 

 carmine ; and for some unaccountable reason scarcely 

 any nucleoli could be found in sections of the stimu- 

 lated ganglion, while they appeared as usual in the 

 ganglion of the other side and control ganglia. A 

 count of the two gave the following : 



Nuclei. Nucleoli- 



, . , ■ A Resting:, 122 92 



Six sections of each contained -j gtimulateJ, 177 28 



The seventh and eighth pairs stained in other ways 

 (Kleinenberg's haematoxylin and by Weigert's method) 

 gave no such result; and in fact the phenomenon 

 could not be made to reappear in any subsequent 

 experiment. Other than this, no results could be made 

 out. 



Three frogs were next taken, each with a control ; 

 each was given the same amount of curare, and the 

 right sciatic nerves of the three were stimulated con- 

 tinuously one, two, and three hours respectively. 

 From the nine stimulated ganglia thus obtained no 

 effect of activity could be discerned. 



An experiment was then made to test the influence 

 of curare upon the working of the central portion of 

 the reflex arc, and the indications seemed quite 

 strong that, although curare does not entirely suspend 

 nervous action in the cord, it does reduce the activity 

 very materially. Its further use was for this reason 

 abandoned.* 



Frog No. 6 was used to demonstrate post mortem 

 changes in the ganglion cells, and does not concern us 

 now. 



' The proof that curare influenced the effect of stimulation is not 

 conclusive, since continuou.s stimulation was also given up at the 

 same time. Further experiment is needed on this point. 



