KLKCTKICAI.r.Y STIMULATED GANCiLION CELLS. 13 



tervals of rest and stiinulatiou being two minutes. 

 Three sets of 100 nuclei each. 



NrcLEi IN //. Cells in li. 



Mean Dlametors. Mena DInmeters. 



i Resting 13.57 Set 1. 39.C9 



t Stimulated . . 12.23 35.00 



_3^ Dili. Til 



S 3 ^ Resting 13. 94 Set 2. 



S 5 - Stiraulatea ..12.56 



a as 



= 1^ DilT. 1.38 



®^i Resting 14.48 Set 3. 



- i Stimulated ..13.26 



Diff. 1.22 

 Sets 1, 2 and 3, volume shrinkage, 24 per cent. 



The volume shrinkage per cent is computed from the 

 mean diameters, treating the nuclei as spheres. 



Besides shrinkage of nuclei, other changes are 

 plainly visible. The protoplasm of the stimulated cells 

 is much more vacuolated than that of the resting, and 

 the staining, by Gaule's quadruple method, is less in- 

 tense. Instead of the coarsely and densely granular 

 constitution of the resting cell, we find the protoplasm 

 of the stimulated cell finely granular and vacuolated. 

 Owing in part to this absence of granules, the nuclei 

 are more distinct in the stimulated cells. In part this 

 is also due to a deeper staining of the nucleus itself, 

 the open reticular appearance of the nucleus giving 

 place to an evenly dense stain. There can be no 

 doubt that in my preparations the stimulated nuclei 

 stain much bluer than the resting, except in cases 

 where pathological conditions were present. 



The five experiments succeeding this were made 

 with the purpose of getting the greatest amount of 

 change possible ; and under the supposition that this 



