18 



Nuclei (four sets 100 each). Cells. 



Mean Dlnmeters Shilnkago. Mean Diameters 



In ^. In Volume. In c 



ist thornclc. Resting 16.29 59-06 



"smio"auid" stimulated.. 14.07 ^H 57.19 



DifE. 2.22 



8th cervical. Resting 14.91 (Selected). All over 50 f. 



Hardened mgtinmiated.. 11.70 51^ (T.)t 



subUmai:. ^^.^ — 



? Tin cervical. Resting 16.60 57.50 



'Stimulated.. 15.41 20^ 56.25 



Flemmlng's 

 lluld. 



Dili. 1.19 



eth cervical. Resting 14.98 44-21 



Hardened in stinj„iated.. 14. 23 14.6^ 44./4 



picric aclu. 



DifE. .75 



Stimulation severe, frequently varied and regulated 

 so as to produce the greatest amount possible of mus- 

 cular contraction in the right fore-leg without giving 

 rise to reflex contractions in other parts of the animal. 

 Muscular contractions in right fore-leg toward close 

 of experiment feeble but constant. Within five min- 

 utes after the animal was bled the muscles of this leg 

 had passed into rigor mortis, the muscles of other 

 limbs being normal and irritable. Pulse and respira- 

 tion remained normal the whole time. 



Besides shrinkage of the nuclei, other important 

 changes occur. For the first thoracic pair, hardened 

 in osmic acid, the nuclei are plump and round in the 

 resting ganglia and stain lighter than the protoplasm. 

 In the stimulated ganglion they are irregular in out- 

 line and stain much darker than the rest of the cell. 

 Especially marked in this case also is the extreme 

 vacuolation of protoplasm in the stimulated cells, and 

 this does not occur in the ganglion of the other side or 



] See explanation page 395, note. 



