KLECTRIOALLY STIMULATED GANGLION CELLS. 



Two Experiments with Stronger Stimulation. 



Cat iVo. 9 (45 seconds a d § £ I | 



rest to 15 seconds S >^ S -: > I 



.„ , work) 2hrs. 100 12.:i9 31^ 40.9^ I 



f« i 10.45 ' 



§1 I CaiiVo. 10 (intervals I 

 o« 15 seconds rest to 



5° [ 15 seconds work). 2 hrs. ion (T.) i:i.S2 23.i);g 32.7?^ 1 



2 12.04 



In the above series tlie stiniulus used was very sliglit, 

 too slight for the most definite results. Still no special 

 study of the sections is necessary to detect the grada- 

 tion expressed in the table, staining as above described. j 

 The use of the one reagent throughout the series 

 has emphasized the fact that osmic acid is not so 

 strongly reduced by the cells of the stimulated ganglia. 

 This sometimes results in so marked a difference in 

 staining that sections of the two ganglia may be easily 

 distinguished by the naked eye. The vacuolation, so 

 striking in the stimulated cells of No. 1 , is only slightly 

 present in these experiments, presumably because the 

 stimuli were not strong enough. 



Many devices were resorted to to eliminate the 

 personal equation and obtain mechanical measure- 

 ments. Three persons unacquainted with the methods 

 of the investigation have kindly consented to assist 

 in the work of measuring. Even here the differences 

 are too marked to make an absolutely neutral state of 

 mind possible, since each of the three, before com- 

 pleting the measurement of a single set, had a very 

 definite notion that the nuclei in the two ganglia were 

 different, in my own iiicasuri'iiiciits 1 was wont from 



