1 8 PURE CULTURES OF 



In Bohemia the beginning was made by Belohoubek, and in 

 Norway by Hejberg. In Denmark my efforts were supported 

 by A. Jorgensen and Gronlund, and considerable activity was 

 displayed especially in Jorgensen's laboratory. In France 

 the matter was first taken up by Louis Marx, and afterwards 

 by Kokosinski, Petit, Monal and others; in England by 

 Wilson, Miller and Hyde ; in America by Wahl and Henius ; 

 in Australia by Peschka. During the last years I have found 

 several able fellow-workers amongst the foreign chemists and 

 botanists who have studied in my laboratory. 



From the above it will be evident that my system carries 

 with it important practical advantages, which may be 

 described as follows : 



1. A definite result and rational working are secured 

 where formerly everything depended more or less upon 

 chance. 



2. Maladies in the beer, causing great losses, are guarded 

 against. 



3. A yeast is obtained having a higher commercial value 

 than the ordinary impure yeast. 



4. Finally, it helps to raise the industry, and this must 

 be a point of great interest to the intelligent brewer. 



A further advantage is that there is no great expense 

 connected with the reform, which is therefore within the reach 

 also of small breweries. In this it therefore differs from some 

 other improvements, such as the introduction of ice machines 

 into breweries. There is also another difference which was 

 overlooked by those who compared the reform brought about 

 by my work with the advantages gained through the intro- 

 duction of ice machines. In the latter case the gain is no 

 more than that which the introduction of ice machines brings 

 to other industries ; the mode of working in the brewery 

 remains the same as before ; our ideas are not enlarged. On 

 the other hand, by the adoption of pure culture, new aspects 

 and a far reaching improvement were introduced. From a 



