u8 TECHNICAL ANALYSIS OF 



it were possible in one way or the other to employ gelatine 

 for such analyses. It is, for instance, often easier, especially 

 for the less practised, to operate with cultivations in gelatine 

 than with cultures in fluids. 



The following was the result of the series of experiments 

 described : After about three days, the contents of both 

 flasks containing respectively the mixtures of 5 cc. water 

 with 5 cc. wort, and 5 cc. water with 5 cc. beer had become 

 cloudy ; they exhibited a very vigorous growth of bacteria, 

 and some yeast-like cells (Pasteur's so-called Tortila) were 

 also present, though in much smaller proportion. After 

 three to four days, several of the drops on the pure gelatine 

 showed growths visible to the naked eye, and similar growths 

 were also found in Koch's gelatine and in the wort gelatine. 

 After four to five days all the drops of the beer and wort 

 mixtures which had been sown on the pure gelatine showed 

 distinct growths ; only in the case of two drops were the 

 above-mentioned yeast-like cells found, and in three drops 

 mould fungi (Penicillium glaucum and Cladosporinm)^ and in 

 all these five drops bacteria were also present. All the 

 remaining drops contained bacteria only. In most cases 

 these growths had liquefied the gelatine. The experiment 

 was discontinued after fourteen days, when it was found that 

 none of the flasks of beer and wort containing the small 

 addition of water showed any signs of a growth. 



In Koch's gelatine there were 1 1 1 colonies, which is equal 

 to 222 calculated on I cc. of water ; all of these contained 

 bacteria, but only a few of the growths had produced lique- 

 faction of the gelatine. The wort gelatine contained 15 

 growths, or 30 calculated on I cc. of water. 



In a second similar series of experiments, the sample of 

 water was taken three days later than in the above. The 

 result was of the same character, but one of the flasks con- 

 taining wort showed a growth of bacteria after four days, and 

 another a growth of Penicfllwm glaiicum after five days. The 



