i 9 2 "DISEA SES" OF BEER, 



Sacch. ellipsoideus II., and C with an equal amount of Sacch. Pastor i- 

 anus III. ; A was not inoculated, but was kept as a control. The yeast 

 was thick, and, as in all the previous experiments, consisted of young 

 vigorous growths which had been cultivated in wort. The experiments 

 having been started in this manner, the casks were placed in the lager 

 cellar of the brewery, and were stored there in the ordinary manner for 

 nearly two and a half months, a comparatively very short storage period 

 for the export beer. The temperature of the cellar was 2 C. 



At the end of the experiment it was found that the 

 strongly infected beer was excellent in every respect, and that 

 its stability was equal to that of the beer which had not been 

 infected. The result in this case was thus the same as in the 

 laboratory experiments with small quantities. 



The above experiments are described in my treatise of 

 1883, which was mentioned above. I will now give some 

 account of the experiments which I have made since then in 

 connection with this disease. 



The experiments which were carried out in the large 

 Pasteur fermenting vessels agree so closely with the conditions 

 obtaining in the brewery that I did not hesitate to apply the 

 results obtained to practical conditions. The only objection 

 which can be raised against this is, that these fermenting 

 vessels differ from those ordinarily employed in the brewery 

 in that the carbonic acid gas cannot as readily escape as 

 under the normal conditions of the brewery. Further, the 

 temperature of the room in which my fermenting vessels 

 stood was slightly higher than is customary in the fermenting 

 cellars of a brewery. It was, therefore, of great advantage to 

 me that the director, Captain Kiihle, gave me a portion of the 

 fermenting cellar at Old Carlsberg for my new experiments. 

 From this time all my practical investigations were tested in 

 the brewery before their completion. The laboratory experi- 

 ments conducted on a small scale can, in fact, only serve as a 

 preliminary guide, and from these alone conclusions cannot 

 be drawn as to what will occur under practical conditions on 

 the large scale. When such experiments are carried out in 



