188 PREPARATION AND MOUNTING 



gradually add the ferrocyanide of potassium dissolved in 

 another ounce, and proceed as before. 



32. Dr. Beale also gives us the following carmine injection 

 to be employed in the same way as the blue.* Take 



Carmine 5 grains. 



Glycerine, with 8 or 10 drops of hydro- 

 chloric acid ^ ounce. 



Glycerine (pure) 1 ounce. 



Alcohol 2 drachms. 



Water 6 drachms. 



Mix the carmine with a few drops of water, and when well 

 incorporated add about five drops of liquor ammonise. To 

 this dark-red solution about half an ounce of the glycerine 

 is to be added, and the whole well shaken in a bottle. 

 Next, very gradually pour in the acid glycerine, frequently 

 shaking the bottle during admixture. Test the mixture 

 with blue litmus-paper, and if not of a very decidedly acid 

 reaction, a few more drops of acid may be added to the 

 remainder of the glycerine and mixed as before. Lastly, 

 mix the alcohol and water very gradually, shaking the bottle 

 thoroughly after adding each successive portion till the 

 whole is mixed. This fluid may be kept ready prepared, 

 and injections made very rapidly with it. 



33. The method of making injections with these colours 

 is the same as with the gelatine mixtures before described, 

 except that no heat is required, and consequently most of 

 the trouble removed. The bottle of the fluid must be well 

 shaken immediately before use; and when the object has 

 been injected, we must allow it to remain in a cool place for 

 a few hours before cutting it. Thin sections of the subject 

 may be cut with Valentin's knife, as before described, and 



* When, however, it is desirable to cut very thin sections of the 

 injected subject, the carmine is sometimes added to a solution of fine 

 gelatine gelatine one part to water eight parts. But the warm water 

 ,nd mode of proceeding which are used with the size solutions before 

 described will be necessary in this case also. 



