206 AVIAN TUBERCULOSIS 



serum tubes inoculated directly from tuberculous lesions in 

 fowls. It grows readily on glycerin agar, Dorset's egg 

 medium, potato and in glycerin bouillon. 



The colonies on glycerin agar vary from one to three 

 millimeters in diameter. The central portion is raised and of 

 a slightly yellowish tint as observed under a hand lens. This 

 central part is surrounded by a flat expansion, about two- thirds 

 the thickness of the center, varying from one-half to one milli- 

 meter in width, with ray-like projections radiating from it and 

 extending into the outer and very thin band of growth with a 

 lobulated margin. On the egg medium of Dorset the growth 

 is not more vigorous than that upon glycerin agar. Potato 

 cultures are quite vigorous, wrinkled and of a yellowish- 

 brown color. 



Fowls inoculated in the abdominal cavity or subcutane- 

 ously with from one-half to one cubic centimeter of a glycerin 

 bouillon culture develop either localized or generalized tuber- 

 culosis in from six weeks to three months, but a much longer 

 time is necessary to destroy them. 



Rabbits and guinea pigs are not readily infected by the 

 inoculation of pure culture. Moore and Ward failed to pro- 

 duce an}' tuberculous lesions in these species. 



§ 151. Morbid anatomy. The lesions are widely dis- 

 tributed, and vary much in their location in different individ- 

 uals. The liver is most frequently involved. The spleen, 

 intestines, mesentery, kidneys, lungs and skin are affected in 

 order mentioned. The appended table gives the distribution 

 of the lesions in 17 cases observed by Moore. 



