DIFFERENTIAL DIAGNOSIS 



261 



3. Actively motile in liquids. 



4. From 3 to 9 flagella are 

 demonstrable. 



5. Vij<orous growth in alkaline 

 nutrient liquids. Less vigorous if 

 liquids are acid in reaction. 



6. Moderate growth on potato. 

 (Varies according to reaction). 



7. Distinct growth on gelatin. 



8. Saponifies milk in from 3 to 

 4 weeks. 



9. Ferments dextrose with the 

 formation of acids and gas. 



10. Does not ferment lactose. 

 Bouillon containing it becomes 

 strongly alkaline. No gas. 



11. Does not ferment saccha- 

 rose. Bouillon containing it be- 

 comes strongly alkaline. No gas. 



12. Destroyed by moist heat at 



58° C. in 15 minutes. 



13. Dies in water in from 2 to 4 

 months. 



14. It dies in the soil in from 2 

 to 3 months. 



15. Rabbits injected subcutane- 

 ously with o.i cc. of a bouillon 

 culture of a virulent bacillus will 

 die in from 5 to 7 days. Enlarged 

 spleen, necrotic foci in liver. 



16. Rabbitsinoculated with cul- 

 ture of an attenuated variety live 

 from lo to 20 days or recover. The 

 lesions are enlarged spleen and 

 infiltration of the follicles in 

 Peyer's patches. 



17. In guinea pigs the lesions 

 are practically the same as in rab- 

 bits. Death occurs in from 7 to 12 

 days. 



3. Not motile in liquids. 



4. No flagella have been found. 



5. (Trowth moderate or feeble 

 in alkaline nutrient liquids. No 

 growth if liquids are acid. 



6. No growth on potato. 



7. Feeble or no growth on 

 gelatin. 



8. Produces no apparent change 

 in milk. 



9. Ferments dextrose with the 

 formation of acids but no gas. 



10. Does not ferment lactose. 

 No gas. 



11. Ferments saccharose with 

 the formation of acids. 



12. Destroyed by moist heat at 

 58° C. in 7 minutes. 



13. Dies in water in from 10 to 

 15 days. 



14. It dies in the soil in from 4 

 to 6 days. 



15. Rabbits injected subcutane- 

 ously with o.oi cc. of a bouillon 

 culture of a virulent bacillus will 

 die in from 16 to 20 hours. 

 Septicemia. 



16. Rabbits inoculated with a 

 culture of an attenuated variety 

 will live from 4 to 10 days. The 

 lesions are local infiltration of pus 

 cells with pleuritis, pericarditis or 

 peritonitis. 



17. Guinea pigs are slightly 

 less susceptible than rabbits. There 

 is more local reaction. Death oc- 

 curs in from i to 4 days. 



