3l8 ACTINOBACILLOSIS 



aration. It stains with the ordinary aniUne dyes.^ It does 

 not take the Gram stain. 



In the fresh tissues or in sections it appears in granules 

 the same as in actinomycosis. 



The actinobacillose is virulent for guinea pigs and rab- 

 bits. When inoculated into the abdominal cavity with pure 



i 



w 



Fig. 79. A pltotoo;rciph of a section of a tuinor stained by Grain's 

 metJwd but not fully decolorized. X about 1,000. {After Higgins). 



*Higgiiis recommends the Romanowsky stain as modified by Dut- 

 ton^and Todd for sections or preparations from pus. The formula for 

 the stain and method for its use are as follows : 



Stain: Eosin, aqueous solution i part 



Borrell's Blue . i " 



Water 8 parts 



Mix just before using and filter. Suspend the preparations (sections 

 fixed to the slide) upside down on the stain to saturate them from 

 below, to avoid precipitate. Stain in this solution for thirty minutes. 

 Wash thoroughly in water, then in a 10% solution of tannic acid, which 

 will^brighten the color, and again wash in water. Dehydrate in alcohol, 

 clear and mount in xylol balsam. The stain as above prepared spoils 

 quickly. 



