394 TRYPANOSOMA 



The nucleus usually divides into equal parts, but may break 

 into several segments. After the nuclear division the proto- 

 plasm may assume various irregular forms. The young nuclei 

 arrange themselves in groups, and the parasite twists and 

 splits by longitudinal or more often by transverse fission 

 (Fig. 104). The new forms resulting from the division soon 

 assume the regular shape. Plimmer and Bradford consider 

 longitudinal and transverse division the more frequent modes 

 of reproduction. They observed conjugation, which con- 

 sisted in the fusion of the micronuclei, followed by an amoeboid 

 stage and division by segmentation. The order of division 

 appears to be (i) centrosome, (2) flagellum, and (3) nucleus 

 and protoplasm. Other forms of reproduction have been 

 described by Martini, Taveran and Mesnil and others. Invo- 

 lution forms have been observed by a number of workers. 

 Rodet and Vallet state that Tr. Brucei multiply principally in 

 the blood and lymph. There is a difference of opinion con- 

 cerning the agglutination of Trypanosoma. Musgrave and 

 Clegg state as a result of their researches that the so-called 

 phenomenon of agglutination is of no value from a diagnostic 

 point of view, and it is too uncertain, if it is a reaction, to 

 serve as an index of immunity or susceptibility. 



§ 306. Distribution in the body. It is the opinion of 

 most students of the Trypanosoma, that in the infected animal 

 they are found in all of the body juices, and are not present at 

 the same time in great numbers in one part, with but few in 

 another. Animals having many parasites in the blood when 

 killed show them also in the organs ; and if they are not 

 demonstrable in a microscopic examination of tlie one they will 

 not appear in the other. The blood of animals suffering from 

 the disease is always infectious by animal inoculation, 

 although the parasites may not be found microscopically at the 

 time. Martini, however, regards the spleen, lymphatics, bone 

 marrow, and to a less extent, the liver and kidneys, as the 

 places for the destruction of Trypanosoma. It has been found 

 that Trypanosoma injected into the peritoneal cavity multipl}^ 

 considerably before they enter the blood. 



