440 RINDERPEST 



ture again drops to normal. This is followed, under the same 

 circumstances, by 500 cc. and again 1000 cc. After this the 

 animal is bled for serum. They are bled with a trocar and 

 canula from the jugular vein. The animals are bled three 

 times at intervals of a week, after which they are injected 

 again with 1000 cc. of virulent blood. 



In using the serum from 50 to 100 cc. should be injected 

 subcutaneously by aseptic methods. 



The advantages of this method of immunizing cattle are : 

 (i) It produces no reaction, (2) it does not check the flow of 

 milk, (3) it confers immunity almost immediately, (4) if 

 the disease has just begun it will modify the attack resulting 

 in a milder form, (5) no deaths follow its use, (6) pregnant 

 animals do not abort, and (7) the serum can be prepared in 

 any quantity and it can be kept for seven or eight months. 



The disadvantages of this method are ( i ) the short period 

 of immunity — 2 to 4 months, (2) skill required to obtain the 

 serum, (3) occasionally, according to Jobling, very little 

 immunity is conferred. 



The other methods that have been tried with more or less 

 success are : inoculation with attenuated virus ; injection of 

 the bile of animals dead of the disease ; injecting the bile 

 associated with virulent liquid ; the injection of the serum 

 from immune animals. 



REFERENCES. 



1. Danvsz, Brodet and Theiler. The Vet. Journal. \o\. 

 XLVI (1898), p. 298. 



2. Edingtox. Report of investigation. T/ie Veterinary Journal, 

 Vol. XLVI (189S), p. 64. 



3. Gamgee. The cattle plague. London. 1866. 



4. Holmes. vSome diseases complicating rinderpest among cattle 

 in India. Jour. Conip. Path, and Thera., Vol. XVII (1904), p. 317. 



5. Jobling. Report of the Director of the Serum Laboratory. 

 Fourth annual report 0/ the Philippine Islands. 1903. Ibid. Bulletin 

 No. 7, Bureau of Government Laboratories. Manila, /90?. 



6. KocH. Report. The Veterinary Journal, \'o\. XLV (1897), 

 p. 204. S\i,o Centralbl f. Bakteriol. Bd. XXI (1897), S. 526. 



