564 DISINFECTION 



organisms were used which are very unlike those for which 

 these disinfectants are now wanted. The practical value of 

 the more recent experiments is greater because they have more 

 generally dealt with species of bacteria with which most of the 

 work of disinfection has to do. 



2. The power of resistance of the same species of bac- 

 terium varies greatly under different conditions. For instance, 

 Bear found that a freshly inoculated culture of the bacterium 

 of diphtheria was destroyed with 1 15000 of nitrate of silver, but 

 that a twenty-four hour culture required i.ioooofthe same 

 agent to kill it in the same space of time. In some work done 

 by Esmarch he made use of anthrax spores from seventeen 

 different sources. They were destroj-ed by steam at 212° F. 

 in from one to twelve minutes and by a five per cent solution 

 of carbolic acid in from two to forty-two days. 



3. The medium in which the bacteria exist influences the 

 results of the disinfectants. The bacterium of tuberculosis 

 from an aqueous suspension dried upon threads may be promptly 

 destro\'ed by mercuric chloride, but in fresh, purulent, 

 tuberculous discharges it cannot be trusted to destroy them. 

 Again Behring says that sporeless anthrax bacteria in water 

 are killed by corrosive sublimate, 1:500,000; in bouillon, by 

 1 140,000 ; but in blood serum not with certainty with a solution 

 of 1:2000. Some disinfectants are influenced very much by 

 the character of the material which contains the infectious 

 organism, while other disinfectants are influenced to a com- 

 paratively slight degree. The experimental work which does 

 not take the influence of the media upon the disinfectant into 

 account is not of much practical value and failures are to be 

 expected in the work of the disinfector who does not act in 

 accordance with this fact. 



4. The temperature under which the disinfecting agent 

 acts influences very much the rapidity and the certainty of its 

 action. Thus Heider found that anthrax spores that survived 

 the action of a five per cent solution of carbolic acid thirty-six 

 days at ordinarj- room temperature, were killed in from one to 



