Edith E. HooaKiNSON 189 



and subsequently learned that in his paper of 1910 Whitney stated that 

 an apparently thelytokous strain could be made to produce arrhenotokous 

 $ $ when fed upon the green flagellate, Chlamydomonas. I found that 

 rich cultures of green protozoa could be made by the following method. 

 Bones and meat are covered with water and allowed to stand for a few 

 days. A little of the solution is put in a glass jar, filled up with 

 spring water and infected with green protozoa — Euglena, etc. This is 

 placed in sunlight and in 3 to 5 days a rich culture is formed and is 

 ready for use. By this means cultures of different ages and in different 

 conditions can be used, if required. 



Experiment I. A thelytokous female, a descendant of XXX in the 

 test given above, was selected, as few arrhenotokous females had occurred 

 in this line, and put in the green protozoa culture. The details of the 

 descendants of XXX when in the horse manure solution are given 



Totals ... 185 9 



* A break occurred here in the continuity of the generations as generations 3 to 7 were 

 bred away from the laboratory. 



These are the details of the descendants of the thelytokous female 

 in the green protozoa culture. 



T A 



AAAAAAAAAAATTTAAAATTAATTTTATAAAATTAAAAA 12 27 

 ATTTATATTTAAAAAATAAATATA 10 14 



A T T T T A 4 2 



13—2 



