I 



A. B. Stout 101 



formed closely to the original description (Hemsley,1905) oiN.Forgetiana, 

 but these were also plants whose flowers were of lighter and of darker 

 shades of red, and there were green-flowered and white-flowered plants. 



It may be noted here that no pure stock of this species was ever 

 kept in culture from the original and only importation of seed obtained 

 from a wild source. In reply to inquiry, the author learns the following 

 by letter from the firm of Sanders of St Albans, England, who made 

 the importation : " We never sold any of the original stock. We simply 

 kept them for hybridizing purposes, afterwards selling the entire lot 

 together with the seed. We had previously secured a batch of really 

 fine new crosses with N. affinis, etc. resulting in a lovely set. When 

 these plants were in full bloom in all the shades of rose, purple, white, 

 pink and crimson the effect was magnificent. We did not continue 

 with the growing of the Nicotianas but sold our stock. We regret very 

 much having lost this remarkable species." 



Series 2. A self-fertile plant of the 1917 crop which appeared to be 

 identical with the original description of N. Forgetiana was the parent of 

 24 plants grown under field conditions. Guarded self-pollinations were 

 made by hand at frequent dates from the first to the very last day of 

 bloom. 



Eleven plants were entirely self-incompatible. They bloomed for 

 periods of fi-om 22 to 65 days, and the total of all flowers selfed was 377. 



One plant was judged as fully self-compatible. It produced fine 

 pods in all flowers selfed on the first, second and fifth days of bloom. 

 No controlled selfings were made thereafter. 



The other 12 plants were partially or feebly self-compatible. The 

 complete data for these would occupy much space, but as the results 

 have a very special bearing on the variations in self-fertility they can 

 be summarized, at least for several plants which are typical for the 

 results. 



One plant began blooming on July 27 and was still blooming on 

 Nov. 6 when there was a heavy frost. A total of 80 flowers was selfed 

 on twenty different dates. On the third day of bloom the fourth flower 

 to open produced a pod with seeds ; 2 pods developed from the 2 flowers 

 that opened on the fourth day ; one of 2 flowers selfed on the twelfth 

 day developed a pod ; 3 selfed on the seventeenth day gave pods ; on 

 Sept. %h, 6 out of 10 flowers selfed gave pods, but on ten dates im- 

 mediately preceding, self-pollinations of 27 flowers all failed. From 

 Sept. 4 until the end of the season about half of the flowei*s selfed 

 produced pods and seeds. In respect to the proportion of flowers which 



Journ. of Gen. ix 7 



