A. B. Stout 105 



variation and not as a phenomenon characteristic of self-incompatible 

 plants. 



Liniim grandifiorum. 



Numerous tests for self-fertility have been made especially at the 

 end of the period of bloom for both long- and short-styled plants of this 

 species. Plants that made a vigorous growth in the field have been cut 

 back, replanted in pots and grown during the winter in a greenhouse. 

 Such plants bloomed profusely in isolation and hundreds of self-pollina- 

 tions were made by hand. All self-pollinations have failed completely. 

 The species appears to be completely self-incompatible. All intra-form 

 crosses which were made failed, but there was always marked fertility 

 in inter-form crosses. The relations in crosses were however not ex- 

 tensively tested. 



Cichorium Intyhus. 



It should perhaps be stated here that the evidence already reported 

 for wide variation in self-compatibility in this species does not involve 

 a mingling of data collected at early and late periods of bloom, nor has 

 there been any comparison between such data. An examination of the 

 records for dates and periods of flowering shows that in no case have 

 controlled pollinations been begun later than the tenth day after the first 

 flower head opened, and that for 95 "/^ of all plants studied pollinations 

 were begun during the first three days of bloom and continued over an 

 interval which seldom extended to the mid-date of bloom. 



In the more robust races, and especially in the variety red-leaved 

 Treviso, the period of blooming often continued until plants were killed 

 by heavy frosts early in November. Toward the end of the period of 

 bloom relatively few flower heads open, and these are rather scattered. 

 During the latter part of September and in October many flower heads 

 open irregularly and poorly, and when plants are killed by frost the seed 

 in many heads is immature. I have therefore never made any pollina- 

 tions of this species later than the 6th of September. It has been most 

 convenient, and it has affbrded a most uniform treatment as well, to begin 

 pollinations within the first three days of bloom and to continue until 

 about the tenth day. Especially for the plants of the variety red-leaved 

 Treviso this covered but a small part of the period of bloom. 



Controlled self-pollinations have been continued for a longer period 

 than was the rule, in the case of certain plants found to be self-compatible 

 and from which considerable seed for planting was desired. Seed pro- 



