present. Six birds were inoculated with each filtrate. Two of 

 them subcutaneously, two submucously and two intraperito- 

 neally. Four birds were used for the four different filtrates. 

 Daily records were taken as to the temperatures, appetite and 

 other clinical symptoms. The birds remained normal until the 

 12th day and from the 12th to the 20th day all of them became 

 sick. All showed an elevation of temperature of two degrees 

 or more, dullness and loss of appetite. Eleven had nasal dis- 

 charge, eye lesions and saliva of a thick consistency. Seven 

 had yellow caseous deposits in the mouth. Of these, three died 

 in a few days and inoculations on media from the heart and 

 liver showed no growth. Six birds showed general symptoms 

 of roup but no local lesions. 



Experiment No. 4 



To determine the action of organisms on the remaining six 

 birds whose resistance had been reduced by the filtrate injection. 

 These birds were injected as follows: 



One submucously with organism No. 1. 

 One submucously with organism No. 2. 



One submucously with organism No. 3. 

 One submucously with staphylococci albi. 

 Two submucously with a mixture of the four organisms 

 mentioned. 

 In all cases typical lesions of the disease appeared at the 

 point of injection. 



Experiment No. 5 



Experiment to transmit the disease by inoculating the 

 mouth deposits of sick birds into healthy ones. 



Six birds were inoculated submucously with scrapings from 

 these lesions and on the third day pustules appeared at the 

 point of injection. On the fifth day a yellowish membrane ap- 

 peared. Lesions disappeared on the tenth day and typical 

 symptoms of the disease were not produced. 



15 



