• •• ■•• • » • # • • * . 



.•...'..'.■•• • • • 



No special characteristics as to cultural growth an.l 

 pathogenicity. 



Organism No. 8. B. necrophorous. 



Ox-ganism No. 9. A colon-like organism. Gram negative. 

 Gelatin and blood serum: not digested. 

 Milk: coagulated. 

 Acid in litmus milk. 

 Lactose broth: acid and gas. 

 Sucrose-broth: acid and gas. 

 Dextrose broth: acid and no gas. 



No indol. Nitrates were reduced to nitrites and am- 

 monia. 

 Organism No. 10. Staphylococcus albus. 

 Organism No. 11. Staphylococcus aureus. 

 Organism No. 12. Micrococcus citreus. 

 Organism No. 13. Sarcina tetragina. 



From the description of the pathogenicity of the different 

 organisms it is seen that none were capable of producing the 

 typical disease symptoms. On injecting an emulsion of all the 

 different organisms more extensive lesions were produced. 



Experiment No. 2 



a. The blood of a series of sick birds was examined by 

 means of a dark-field illuminating apparatus and no 

 organism was noted. 



b. Inoculations from the heart, blood and liver substance 

 showed no growth. 



c. A series of birds were injected subcutaneously, submu- 

 cously, intravenously and the wattles scarified with 

 amounts varying from .2 to 1 cc. of blood serum from 

 sick birds. 



The disease was not produced. 



Experiment No. 3 



Four birds with all the clinical symptoms of the disease 

 were killed and an emulsion of the heads of these birds, passed 

 through a Berkefeld filter, were used for this experiment. Each 

 head was macerated and triturated separately with sterile sand 

 and sterile physiological salt solution. The emulsion of each 

 head was separately filtered through a Berkefeld filter. The 

 filtrates were tested out on culture media and no growth was 



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