30 



ESTABLISHMENT OF VARIETIES IN COLEUS 



mixed and irregular patterns like those that appeared from patterns 

 treated in tables 3 and 4. 



There were 16 cases of bud variation; 5 were concerned with the red 

 epidermis, 2 giving solid red, and 3 giving no red. There were 2 cases 

 of vspontaneous appearance of yellow, giving branches sectorial for the 

 green-yellow spotted-red blotched pattern. There were, also, 9 cases of 

 spontaneous development of j^ellow that were not carried on in suc- 

 cessive leaves, and with the exception of 1 case were confined to but 

 one or two leaves. These yellow blotches were large, irregular-shaped 

 pure-yellow areas covering from one-eighth to one-fourth the entire 

 area of a leaf. The locations and relative sizes of these yellow blotches 

 in the leaves of one plant are shown in text-figure 1, the shaded protions 

 of which indicate yellow areas. All other leaves were pure green and 

 the branches produced in the axils of the yellow-blotched leaves were 

 pure green. 



Text-figure 1. — Position of the yellow areas that developed spontaneously in these 



leaves of plant 11714221. 



A study of pedigrees reveals the interesting fact that 6 of the plants 

 with spontaneous yellow all descended from a branch on plant 11714. 

 The full record of this clone is given in table 13, but the summary of 

 the pedigrees of the particular line of descent is here given in table 6. 



The pedigree numbers enable one to trace relationship quite readil3^ 

 In this case we note that plant 1171 gave a bud variation with loss of 

 yellow. Plant 11714 was grown as a chimera with about one-half pure 

 green. From the green part two cuttings were made for plants 117142 

 and 117144, both of which remained constant for loss of yellow. In 

 September 1913, three cuttings were made and from two of the plants 

 grown three more cuttings were taken in the following spring. From 

 September 1912 until August 1914 all plants grown in these lines of 

 descent were constant for loss of yellow, then in one season the spon- 

 taneous development of yellow occured in 6 closely related plants. 



