reagent, which may have caused a ewelllng of the chromosomes. 

 This result was not always obtained however hy the same re- 

 agent . 



when de staining Is carried so far as to remove all the 

 stain from the cytoplasm and the achromatic figure, the equa- 

 torial plate may be shown to have a structure like that repre- 

 sented in Pig. 16a. This was drawn from a section cut from 

 the edge of the plate. Here it appears that the chromosoines 

 are stretched as they are pulled apart. Strands of chromatin 

 pulled out between the separating groups of chromosomes may 

 be seen In the later stages of the anaphase. By more exten- 

 sive destainlng we may obtain what appears to be only the 

 cores of the chromosomes as shown in Pig. 16b. 



In Pigs. 17 to 22 various stages in the anaphase are rep- 

 resented. The interzonal fibers and the mid-body axe very 

 distinct In Pigs. 19 to 21. 



The second mitotic division follows very soon after the 

 first. The chromosomes become somewhat separated and are 

 then drawn together again Into the equatorial plate ready 

 for the second division (Pigs. 23 to 25). Plgure 26 shows 

 the beginning, and Pig. 27, the end of the anaphase. Here 

 again the Interzonal fibers and the mid-body are distinctly 

 seen and a portion of the cytoplasm is definitely associated 

 with each daughter nucleus. The nucleus of the spermatid 

 Is now organized and persists in a sort of resting condition 

 for a comparatively long time. The centrosome may also be 

 distinguished for a considerable time but later I was unable 



