13 



ond vesicle now forms. (Pig. 59). These two vesicles "become 

 transformed Into a tu"bule containing the central body. This 

 tuhule will hereafter be spoken of, as the inner tubule . At 

 its outer end a ring of darkly staining substance is found (Pig. 

 60). This seems to have* been derived from the central body. At 

 least a study of Pigs. 54 to 60 may well suggest such an inter- 

 pretation. The central body finally becomes reduced in diameter 

 and appears to be a solid rod. It is not stained by thionin, 

 nor by safranin,but is readily stained with iron-haematoxylin. 



The inner tubule is stained green with safranin coimter 

 stained with Lichtgriin; blue with thionin counter stained with 

 eosin; and black with iron-haematoxylin. 



During this whole period the content of the capsule shows 

 an increasing affinity for chromatin stains. It is colored 

 brown with iron-haematoxylin. In some series a sort of ring 

 shaped cloud appears in the capsular contents. At first it is 

 near the outer wall but gradually it contracts towards the ves- 

 icle at the end of the central body and finally settles in the 

 wall of the tubule when that structure takes its final form. 

 With Delafield's haematoxylin the contents of the capsule is 

 readily stained and with safranin it takes a dull red color. 

 In the early stages of development the content of the capsule 

 is stained green when the preparation is treated with the 

 safranin and Llchtgrun combination, but in the later stages 

 the green is masked by the red. In stages represented in 

 Pigs. 53 to 55 a sort of foam or alveolar structure can some- 

 times be observed in this substance. 



While the capsule and the structures within it are assum- 



