34 



to find these minute structures in the relatively immense egg. 

 After one has heGorne familiar with these structures and their 

 position on the egg it is possible to find them quite readily 

 with other stains such as Delafield's haematoxylin, iron- 

 haematoxylin, or safranin and Liohtgrun. 



The relation of the sperms to eggs taken from the lumen 

 of the ovary is shown in Pigs. 88 to 92. Sperms in the same 

 condition are also found In eggs taken from the oviduct as 

 is shown in Pigs. 9Z and 94. How when these figures are com- 

 pared with Pigs. 96 and 87, which have been treated with the 

 same fixing reagents, it is evident that it is the everted 

 portion of the sperm that has gone through the shell of the 

 egg. The nuclear cup (n.c. Pigs. 91 and 92) is on the out- 

 side of the shell. The everted tuhule forms a vesicle with- 

 in which one sees the Inverted capsule (inv.c. Pig. 91). Hera- 

 after I shall call this everted tuhule and capsule, the sperm- 

 vesicle. At the inner-end of this sperm-vesicle the ejected 

 central hody may he seen (Pigs. 88, 91 to 94 c.b.). That the 

 part which remains outside is the nuclear cup with its radiat- 

 ing pseudopodla can he more clearly seen hy a surface view of 

 the structure as it lies upon the eg^. Such a view is present- 

 ed in Pig. 95. Furthermore the staining reactions are in ac- 

 cord with those observed in the mat:ire sperm and the artificial- 

 ly exploded ones and are as follows: 



) the part outside of the shell-blue. 

 Thionin and eosin ( 



) the part inside of the shell--red. 



„ (part outside of shell--red 

 Safranin and Liohtgrun ) 



(part inside, green mixed with red. 



