48 Investigations on the Life-History 



The non-liquefying forms were obtained in numbers which, of course, 

 coincide with the differences between the figures given in Charts IV. 

 and V. Almost nil in May and June, the summer increase is most 

 marked in the oesophagus, and proportionally in the intestine, when 

 compared with the last chart; while the autumn decrease is greatest in 

 the oesophagus, less in the stomach, and least in the intestine, from 

 which, indeed, the same number of growths were obtained as in the 

 months of July and August. 



(c) Kelts. . 



Cultivations were made from the intestinal tract of one kelt, 

 caught in July 1895 in the upper reaches. Owing to the examination 

 of only a single specimen of this class of fish, the results have been 

 included in the preceding section. 



A large number of non-liquefying growths were obtained from the 

 esophagus, of both liquefying and non-liquefying from the stomach and 

 intestine, the latter section containing a few bacilli coli communes. The 

 peptic activity of 'this fish was nil, the amount of albumen digested by 

 an extract of the intestine, 12-6 per cent. 



(d) Differences Between the Numbers of Micro-Organisms Grown from the 

 Alimentary Tract in Salmon caught in the Estuary and of those 

 caught in the Upper Parts of the Rivers. 



In the 12 fish from the upper waters, the following numbers of 

 organisms were counted : 



Total, 

 Per Fish, 



in the 29 caught in the estuaries 



and in the total number of fish, i.e., 41 



Total, 

 Per Fish, 



5,011 4,544 



122-2 110-8 



3,616 



13,171 

 321 



In the total number of fish examined the oesophagus contained the 

 largest number of organisms, the intestine the least. 



On the other hand, the colonies grown from the stomach contents of 

 the fish caught in the upper reaches were the most numerous, then the 

 colonies from the oesophagus, and those from the intestine. 



The colonies obtained from the oesophagus in fish from the estuaries 

 presented a marked excess in numbers over those from the stomach 

 and intestines. The numbers in the latter were nearly equal. 



[TABLE. 



