of the, Salmon in Fresh Water. 99 



beginning of August the intestinal fat has almost disappeared. This 

 observation, of course, applies only to fish in the upper waters. The 

 state of the intestinal fat in fish in the lower waters has not, so far as 

 I am aware, been studied. 



In every fish after Number 20 a note of the fat on the appendages 

 was kept, and the following table gives the results of these observa- 

 tions: 



TABLE v. 



* Two of these, 72 and 79, were "winter salmon" and are not included in the percentage table 

 below. 



This gives the percentage of fish as follows : 

 TABLE VI. 



In order more accurately to show the extent of the change in the 

 intestinal fat a series of analyses were made from fish at the mouth and 

 from fish in the upper reaches of the rivers throughout the season. The 

 examples selected for this were average specimens. 



METHOD. 



The pyloric appendages with the upper part of the intestine were 

 preserved in spirit. They were then chopped up, and repeatedly ex- 

 tracted with hot alcohol, the alcohol being filtered hot. The residue 

 was then repeatedly extracted with ether. The alcoholic residue was 

 dried over the water bath, and repeatedly extracted with ether, the 

 ether being united with that from the residue. The ether was dis- 

 tilled off, and the fats weighed in the usual manner. 



Table VII. gives the results of these analyses : 



[TABLE. 



